Expansion of a Zebrafish Research Core Facility

斑马鱼研究核心设施的扩建

基本信息

批准号：
8713039
负责人：
DAVID J. GRUNWALD
金额：
$ 50万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2016-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8713039
关键词：
Address Animal Housing Animal Model Animal Welfare Act Animals Applications Grants Area Award Biomedical Research Caring Cells Collaborations Communities Core Facility Development Direct Costs Educational process of instructing Environment Equilibrium Equipment Faculty Fishes Fluorescence Microscopy Funding Funding Agency Funding Mechanisms Future Gene Targeting Genes Genetic Grant Health Sciences Heart Diseases Housing Individual Journals Knock-out Laboratories Laboratory Animals Malignant Neoplasms Metabolism Methods Microscope Microscopy Monitor Muscular Dystrophies Nature Neurons Palpable Play Policies Positioning Attribute Procedures Production Publications Publishing Quality of Care Quarantine Recruitment Activity Research Research Personnel Research Project Grants Research Support Resources Rest Role Science Secure Supervision System Technology Time Training Transgenic Animals Transgenic Organisms United States National Institutes of Health Universities Utah Vision Water Supply Work Zebrafish animal facility animal resource base career design egg experience innovation instrumentation interest member mutant nervous system development new technology novel pathogen programs research facility response square foot success synergism

项目摘要

DESCRIPTION (provided by applicant): We seek funds to renovate and expand the Centralized Zebrafish Animal Resource (CZAR), the Core Facility for Zebrafish Research at the University of Utah (U of U). Research with zebrafish (Danio rerio) is a significant focus of biomedical research at the U of U. Our investigators use this model organism to uncover mechanisms responsible for heart disease, cancers, muscular dystrophies, response to pathogens, development of the nervous system, and other medically important conditions. More than 20 faculty members from eight Departments currently use the CZAR. Research performed in the CZAR has been supported by major biomedical funding agencies, including NIH, HHMI, ACS, AHA, MDA, and March of Dimes, and is currently supported by ~$7,800,000 in annual direct cost funding from grants. Pending grant applications (as of July, 2013) request an additional ~$2,000,000 in annual direct cost funding. Research performed in the CZAR has been published in top-tier biomedical journals, including (past 5-6 years) Science, Nature, Cell, Nature Genetics, Nature Methods, Neuron, Cell Metabolism, Developmental Cell, PNAS (USA), PLoS Genetics, Genes & Development, and Development. Thus, the facility we seek to expand facilitates cutting edge biomedical research and plays a highly significant role in supporting biomedical research at the U of U. The CZAR was established in 2001 with matching funds from the U of U and the NIH through the G20 funding mechanism. It was designed to support the research programs of five early-to-mid-career investigators. In a bold and innovative move, in 2002 the CZAR became an open-access shared-use Core Facility serving the entire U of U community, receiving yearly operational support and periodic instrumentation funds from the U of U. In addition to animal housing, care, and uniform oversight, as a Core Facility the CZAR provides: 1) space and equipment for experimental procedures; 2) teaching and expertise; 3) access to sophisticated technologies; and 4) opportunities for collaborations among multiple research groups. As best we know, the CZAR was the first University Core Facility for Zebrafish Research in the US. The Core has attracted many established researchers at the U of U to begin working with the zebrafish and has become the basis of our ability to recruit many junior zebrafish investigators to the Health Sciences Center at the U of U (five new faculty in the past four years). The CZAR is in desperate need of expansion. More than 80 researchers of all levels currently utilize the Core, which has been operating at 90+% housing capacity for the past 5 years. Newly recruited faculty, recently awarded grants from the NIH "Programs to Enhance Zebrafish Research" (PAR-11-130 and -131), and the "Director's New Innovator Award Program" (RFA-RM-12-016) funded to generate new mutants and transgenic lines for use by the worldwide zebrafish research community, and new research opportunities afforded by the gene knock-out and gene-targeting technologies recently developed at the U of U require increased housing and experimental space in the CZAR. The current CZAR comprises 3646 sq ft; it contains 70 6- shelf 60" racks (5040 3-liter tanks) for standard housing of zebrafish, and one rack for holding animals in quarantine. The proposed $1,129,380 project will expand the capacity of the CZAR by over 50%. We request $500,000 from the NIH; the rest will be provided by the U of U. The specific aims of this project are to: 1) Renovate 1,923 sq ft of existing building space that is immediately adjacent to the current CZAR to expand the current CZAR; 2) Purchase and install 36 new 6-shelf 60" racks and thus increase zebrafish the housing capacity in the CZAR by 51%; 3) Double our quarantine capacity; and 4) Create new experimental procedure areas, including a room for off-cycle production of eggs. The project addresses several objectives of the FOA. It efficiently accommodates demonstrated needs for conducting PHS-funded biomedical research. Because it functions as a shared-use Core Facility, the facility can be used efficiently to accommodate the changing needs of individual research projects. By expanding zebrafish housing capacity at the U of U within the purview of the current CZAR Core Facility, which is operated under the supervision of a full-time, highly experienced profession staff, the project guarantees the highest quality of care to animals and rigorous compliance with USDA Animal Welfare Act and the DHHS policies related to the care and use of laboratory animals. Finally, the proposed renovation project has employed sustainable design technologies and design approaches throughout.

描述（由申请人提供）：我们寻求资金来翻新和扩大Zebrafish动物资源（CZAR），这是犹他大学斑马鱼研究的核心设施（U O of U）。与斑马鱼（Danio Rerio）的研究是U的生物医学研究的重要重点。我们的研究人员使用该模型生物体来揭示负责心脏病，癌症，肌肉营养不良的机制，对病原体的反应，神经系统的发展，神经系统的发展以及其他重要的医学重要条件。目前，来自八个部门的20多名教职员工使用沙皇。在沙皇进行的研究得到了主要的生物医学资助机构的支持，包括NIH，HHMI，ACS，AHA，MDA和DIMES MARCH，目前得到赠款的年度直接成本资金约7,800,000美元的支持。待定赠款申请（截至2013年7月）要求额外的年度直接成本资金额外约2,000,000美元。在沙皇进行的研究已发表在顶级生物医学期刊上，包括（过去5 - 6年）科学，自然，细胞，自然遗传学，自然方法，神经元，细胞代谢，发育细胞，PNAS，PNAS，PNAS（美国），PLOS遗传学，基因与发展和发展。因此，我们寻求扩大的设施促进了最前沿的生物医学研究，并在美国U的生物医学研究中发挥了非常重要的作用，CZAR于2001年建立了U和NIH通过G20融资机制的匹配资金。它旨在支持五名早期至中型研究人员的研究计划。以大胆而创新的举动，2002年，沙皇成为一个开放式共享用途的核心设施，为U的整个U社区提供服务，从U的U of U. U获得了年度运营支持和定期仪器资金，除了动物住房，护理和统一的监督外，还可以作为核心设施，作为CZAR的核心设施：CZAR提供：1）空间和设备进行实验过程； 2）教学和专业知识； 3）访问精致技术； 4）多个研究小组之间合作的机会。众所周知，沙皇是美国斑马鱼研究的第一个大学核心设施。该核心吸引了U的U of U of U of U of U of U，开始与斑马鱼合作，并已成为我们将许多初级斑马鱼调查人员招募到U的U级卫生科学中心的能力的基础（过去四年来五个新教师）。沙皇迫切需要扩张。目前，所有级别的80多名研究人员都利用了核心，在过去的5年中，住房能力一直以90％+％的住房运行。新招募的教师，最近获得了NIH“增强斑马鱼研究计划”的赠款（第11-1-130和-131页），以及“导演的新创新者奖计划”（RFA-RMMM-12-016），资助用于产生新的Zegrafish Research Communition和Genity Indery Commenity的新型突变体和转基因研究，以及基因的基因研究机会，并获得了基因策略，并获得了基因策略，并获得了基因策略，并将最近在U的U中开发的需要增加沙皇的住房和实验空间。当前的沙皇包括3646平方英尺；它包含70个6架60英寸的架子（5040 3升储罐），用于斑马鱼的标准外壳，一个用于在隔离区持有动物的机架。提议的1,129,380美元的项目将将胸部的项目扩大到50％以上。我们要求NIH的500,000美元。现有的建筑空间与当前的沙皇相邻，以扩大当前的沙皇； 3）我们的隔离能力加倍； 4）创建新的实验程序区域，包括一个卵子生产的空间。该项目解决了FOA的几个目标。它有效地适应了进行PHS资助的生物医学研究的需求。由于它是共享用途的核心设施，因此可以有效地使用该设施来满足各个研究项目的不断变化的需求。通过在CZAR核心设施的范围内扩大U的斑马鱼住房能力，该设施由全职，经验丰富的职业员工的监督进行操作，该项目确保了对动物的最高质量护理，并严格遵守USDA Animal Felfare Act和DHHS政策，以及与实验室动物的护理和使用有关的政策。最后，拟议的翻新项目一直采用可持续的设计技术和设计方法。