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Expansion of a Zebrafish Research Core Facility

斑马鱼研究核心设施的扩建

基本信息

批准号：
8713039
负责人：
DAVID J. GRUNWALD
金额：
$ 50万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2016-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8713039
关键词：
Address Animal Housing Animal Model Animal Welfare Act Animals Applications Grants Area Award Biomedical Research Caring Cells Collaborations Communities Core Facility Development Direct Costs Educational process of instructing Environment Equilibrium Equipment Faculty Fishes Fluorescence Microscopy Funding Funding Agency Funding Mechanisms Future Gene Targeting Genes Genetic Grant Health Sciences Heart Diseases Housing Individual Journals Knock-out Laboratories Laboratory Animals Malignant Neoplasms Metabolism Methods Microscope Microscopy Monitor Muscular Dystrophies Nature Neurons Palpable Play Policies Positioning Attribute Procedures Production Publications Publishing Quality of Care Quarantine Recruitment Activity Research Research Personnel Research Project Grants Research Support Resources Rest Role Science Secure Supervision System Technology Time Training Transgenic Animals Transgenic Organisms United States National Institutes of Health Universities Utah Vision Water Supply Work Zebrafish animal facility animal resource base career design egg experience innovation instrumentation interest member mutant nervous system development new technology novel pathogen programs research facility response square foot success synergism

项目摘要

DESCRIPTION (provided by applicant): We seek funds to renovate and expand the Centralized Zebrafish Animal Resource (CZAR), the Core Facility for Zebrafish Research at the University of Utah (U of U). Research with zebrafish (Danio rerio) is a significant focus of biomedical research at the U of U. Our investigators use this model organism to uncover mechanisms responsible for heart disease, cancers, muscular dystrophies, response to pathogens, development of the nervous system, and other medically important conditions. More than 20 faculty members from eight Departments currently use the CZAR. Research performed in the CZAR has been supported by major biomedical funding agencies, including NIH, HHMI, ACS, AHA, MDA, and March of Dimes, and is currently supported by ~$7,800,000 in annual direct cost funding from grants. Pending grant applications (as of July, 2013) request an additional ~$2,000,000 in annual direct cost funding. Research performed in the CZAR has been published in top-tier biomedical journals, including (past 5-6 years) Science, Nature, Cell, Nature Genetics, Nature Methods, Neuron, Cell Metabolism, Developmental Cell, PNAS (USA), PLoS Genetics, Genes & Development, and Development. Thus, the facility we seek to expand facilitates cutting edge biomedical research and plays a highly significant role in supporting biomedical research at the U of U. The CZAR was established in 2001 with matching funds from the U of U and the NIH through the G20 funding mechanism. It was designed to support the research programs of five early-to-mid-career investigators. In a bold and innovative move, in 2002 the CZAR became an open-access shared-use Core Facility serving the entire U of U community, receiving yearly operational support and periodic instrumentation funds from the U of U. In addition to animal housing, care, and uniform oversight, as a Core Facility the CZAR provides: 1) space and equipment for experimental procedures; 2) teaching and expertise; 3) access to sophisticated technologies; and 4) opportunities for collaborations among multiple research groups. As best we know, the CZAR was the first University Core Facility for Zebrafish Research in the US. The Core has attracted many established researchers at the U of U to begin working with the zebrafish and has become the basis of our ability to recruit many junior zebrafish investigators to the Health Sciences Center at the U of U (five new faculty in the past four years). The CZAR is in desperate need of expansion. More than 80 researchers of all levels currently utilize the Core, which has been operating at 90+% housing capacity for the past 5 years. Newly recruited faculty, recently awarded grants from the NIH "Programs to Enhance Zebrafish Research" (PAR-11-130 and -131), and the "Director's New Innovator Award Program" (RFA-RM-12-016) funded to generate new mutants and transgenic lines for use by the worldwide zebrafish research community, and new research opportunities afforded by the gene knock-out and gene-targeting technologies recently developed at the U of U require increased housing and experimental space in the CZAR. The current CZAR comprises 3646 sq ft; it contains 70 6- shelf 60" racks (5040 3-liter tanks) for standard housing of zebrafish, and one rack for holding animals in quarantine. The proposed $1,129,380 project will expand the capacity of the CZAR by over 50%. We request $500,000 from the NIH; the rest will be provided by the U of U. The specific aims of this project are to: 1) Renovate 1,923 sq ft of existing building space that is immediately adjacent to the current CZAR to expand the current CZAR; 2) Purchase and install 36 new 6-shelf 60" racks and thus increase zebrafish the housing capacity in the CZAR by 51%; 3) Double our quarantine capacity; and 4) Create new experimental procedure areas, including a room for off-cycle production of eggs. The project addresses several objectives of the FOA. It efficiently accommodates demonstrated needs for conducting PHS-funded biomedical research. Because it functions as a shared-use Core Facility, the facility can be used efficiently to accommodate the changing needs of individual research projects. By expanding zebrafish housing capacity at the U of U within the purview of the current CZAR Core Facility, which is operated under the supervision of a full-time, highly experienced profession staff, the project guarantees the highest quality of care to animals and rigorous compliance with USDA Animal Welfare Act and the DHHS policies related to the care and use of laboratory animals. Finally, the proposed renovation project has employed sustainable design technologies and design approaches throughout.

描述（由申请人提供）：我们寻求资金来翻新和扩大集中斑马鱼动物资源（CZAR），这是犹他大学（U of U）斑马鱼研究的核心设施。斑马鱼（Danio rerio）研究是美国大学生物医学研究的一个重要焦点。我们的研究人员利用这种模型生物来揭示导致心脏病、癌症、肌肉萎缩症、病原体反应、神经系统发育和其他医学重要病症的机制。目前有来自八个系的 20 多名教职人员使用 CZAR。 CZAR 进行的研究得到了主要生物医学资助机构的支持，包括 NIH、HHMI、ACS、AHA、MDA 和 March of Dimes，目前每年获得约 7,800,000 美元的直接成本资助。待决拨款申请（截至 2013 年 7 月）要求额外提供约 2,000,000 美元的年度直接成本资金。 CZAR 进行的研究已发表在顶级生物医学期刊上，包括（过去 5-6 年）《Science》、《Nature》、《Cell》、《Nature Genetics》、《NatureMethods》、《Neuron》、《Cell Metabolism》、《Developmental Cell》、《PNAS》（美国）、《PLoS Genetics》、《Genes & Development》和《Development》。因此，我们寻求扩建的设施促进了尖端生物医学研究，并在支持大学生物医学研究方面发挥着非常重要的作用。CZAR 成立于 2001 年，通过 G20 资助机制获得了大学和 NIH 的配套资金。它旨在支持五名早期到中期职业研究人员的研究项目。 2002 年，CZAR 采取了大胆创新的举措，成为一个开放共享的核心设施，为整个 U 大学社区提供服务，每年获得 U 大学的运营支持和定期仪器资金。除了动物饲养、护理和统一监督之外，CZAR 作为核心设施还提供：1）用于实验程序的空间和设备； 2）教学和专业知识； 3）获得先进技术； 4）多个研究小组之间合作的机会。据我们所知，CZAR 是美国第一个斑马鱼研究大学核心设施。该核心吸引了犹大大学的许多知名研究人员开始研究斑马鱼，并成为我们为犹他大学健康科学中心招募许多初级斑马鱼研究人员的能力的基础（过去四年新增了五名教员）。沙皇迫切需要扩张。目前有 80 多名各级研究人员使用 Core，在过去 5 年里，该核心的住房容量一直保持在 90% 以上。新招募的教师，最近获得了 NIH“增强斑马鱼研究计划”（PAR-11-130 和 -131）和“主任新创新者奖计划”（RFA-RM-12-016）的资助，以产生新的突变体和转基因系供全世界斑马鱼研究界使用，以及基因敲除和基因靶向技术提供的新研究机会大学最近开发的项目需要在沙皇区增加住房和实验空间。目前的 CZAR 面积为 3646 平方英尺；它包含 70 个 6 层 60 英寸的架子（5040 个 3 升水箱），用于斑马鱼的标准饲养，以及一个用于隔离动物的架子。拟议的 1,129,380 美元项目将使 CZAR 的容量扩大 50% 以上。我们向 NIH 请求 500,000 美元；其余部分将由美国大学提供。该项目的具体目标是： 1) 翻新1,923 平方英尺紧邻当前沙皇区的现有建筑空间，以扩大当前沙皇区； 2) 购买并安装36个新的6层60英寸鱼架，从而将沙皇区斑马鱼的饲养能力增加51%； 3）将我们的检疫能力提高一倍； 4) 创建新的实验程序区域，包括用于非循环鸡蛋生产的房间。该项目致力于实现 FOA 的几个目标。它有效地满足了进行 PHS 资助的生物医学研究的已证实的需求。由于它作为共享核心设施发挥作用，因此可以有效地利用该设施来满足各个研究项目不断变化的需求。通过在当前 CZAR 核心设施的范围内扩大斑马鱼饲养能力，该设施由经验丰富的全职专业人员监督运营，该项目保证了对动物的最高质量的护理，并严格遵守美国农业部动物福利法和与实验动物护理和使用相关的 DHHS 政策。最后，拟议的改造项目自始至终采用了可持续设计技术和设计方法。