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Expansion of a Zebrafish Research Core Facility

斑马鱼研究核心设施的扩建

基本信息

批准号：
8713039
负责人：
DAVID J. GRUNWALD
金额：
$ 50万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2016-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8713039
关键词：
Address Animal Housing Animal Model Animal Welfare Act Animals Applications Grants Area Award Biomedical Research Caring Cells Collaborations Communities Core Facility Development Direct Costs Educational process of instructing Environment Equilibrium Equipment Faculty Fishes Fluorescence Microscopy Funding Funding Agency Funding Mechanisms Future Gene Targeting Genes Genetic Grant Health Sciences Heart Diseases Housing Individual Journals Knock-out Laboratories Laboratory Animals Malignant Neoplasms Metabolism Methods Microscope Microscopy Monitor Muscular Dystrophies Nature Neurons Palpable Play Policies Positioning Attribute Procedures Production Publications Publishing Quality of Care Quarantine Recruitment Activity Research Research Personnel Research Project Grants Research Support Resources Rest Role Science Secure Supervision System Technology Time Training Transgenic Animals Transgenic Organisms United States National Institutes of Health Universities Utah Vision Water Supply Work Zebrafish animal facility animal resource base career design egg experience innovation instrumentation interest member mutant nervous system development new technology novel pathogen programs research facility response square foot success synergism

项目摘要

DESCRIPTION (provided by applicant): We seek funds to renovate and expand the Centralized Zebrafish Animal Resource (CZAR), the Core Facility for Zebrafish Research at the University of Utah (U of U). Research with zebrafish (Danio rerio) is a significant focus of biomedical research at the U of U. Our investigators use this model organism to uncover mechanisms responsible for heart disease, cancers, muscular dystrophies, response to pathogens, development of the nervous system, and other medically important conditions. More than 20 faculty members from eight Departments currently use the CZAR. Research performed in the CZAR has been supported by major biomedical funding agencies, including NIH, HHMI, ACS, AHA, MDA, and March of Dimes, and is currently supported by ~$7,800,000 in annual direct cost funding from grants. Pending grant applications (as of July, 2013) request an additional ~$2,000,000 in annual direct cost funding. Research performed in the CZAR has been published in top-tier biomedical journals, including (past 5-6 years) Science, Nature, Cell, Nature Genetics, Nature Methods, Neuron, Cell Metabolism, Developmental Cell, PNAS (USA), PLoS Genetics, Genes & Development, and Development. Thus, the facility we seek to expand facilitates cutting edge biomedical research and plays a highly significant role in supporting biomedical research at the U of U. The CZAR was established in 2001 with matching funds from the U of U and the NIH through the G20 funding mechanism. It was designed to support the research programs of five early-to-mid-career investigators. In a bold and innovative move, in 2002 the CZAR became an open-access shared-use Core Facility serving the entire U of U community, receiving yearly operational support and periodic instrumentation funds from the U of U. In addition to animal housing, care, and uniform oversight, as a Core Facility the CZAR provides: 1) space and equipment for experimental procedures; 2) teaching and expertise; 3) access to sophisticated technologies; and 4) opportunities for collaborations among multiple research groups. As best we know, the CZAR was the first University Core Facility for Zebrafish Research in the US. The Core has attracted many established researchers at the U of U to begin working with the zebrafish and has become the basis of our ability to recruit many junior zebrafish investigators to the Health Sciences Center at the U of U (five new faculty in the past four years). The CZAR is in desperate need of expansion. More than 80 researchers of all levels currently utilize the Core, which has been operating at 90+% housing capacity for the past 5 years. Newly recruited faculty, recently awarded grants from the NIH "Programs to Enhance Zebrafish Research" (PAR-11-130 and -131), and the "Director's New Innovator Award Program" (RFA-RM-12-016) funded to generate new mutants and transgenic lines for use by the worldwide zebrafish research community, and new research opportunities afforded by the gene knock-out and gene-targeting technologies recently developed at the U of U require increased housing and experimental space in the CZAR. The current CZAR comprises 3646 sq ft; it contains 70 6- shelf 60" racks (5040 3-liter tanks) for standard housing of zebrafish, and one rack for holding animals in quarantine. The proposed $1,129,380 project will expand the capacity of the CZAR by over 50%. We request $500,000 from the NIH; the rest will be provided by the U of U. The specific aims of this project are to: 1) Renovate 1,923 sq ft of existing building space that is immediately adjacent to the current CZAR to expand the current CZAR; 2) Purchase and install 36 new 6-shelf 60" racks and thus increase zebrafish the housing capacity in the CZAR by 51%; 3) Double our quarantine capacity; and 4) Create new experimental procedure areas, including a room for off-cycle production of eggs. The project addresses several objectives of the FOA. It efficiently accommodates demonstrated needs for conducting PHS-funded biomedical research. Because it functions as a shared-use Core Facility, the facility can be used efficiently to accommodate the changing needs of individual research projects. By expanding zebrafish housing capacity at the U of U within the purview of the current CZAR Core Facility, which is operated under the supervision of a full-time, highly experienced profession staff, the project guarantees the highest quality of care to animals and rigorous compliance with USDA Animal Welfare Act and the DHHS policies related to the care and use of laboratory animals. Finally, the proposed renovation project has employed sustainable design technologies and design approaches throughout.

描述（由申请人提供）：我们寻求资金来翻新和扩展中央斑马鱼动物资源（CZAR），这是犹他大学（U of U）斑马鱼研究的核心设施。斑马鱼（Danio rerio）研究是美国大学生物医学研究的一个重要焦点。我们的研究人员使用这种模式生物来揭示心脏病、癌症、肌肉萎缩症、对病原体的反应、神经系统的发育和其他医学上重要的疾病的机制。来自8个部门的20多名教职员工目前使用CZAR。在CZAR进行的研究得到了主要生物医学资助机构的支持，包括NIH， HHMI， ACS， AHA， MDA和March of Dimes，目前每年从赠款中获得约780万美元的直接成本资助。待决拨款申请（截至2013年7月）要求额外的每年约200万美元的直接成本资金。在CZAR进行的研究已在顶级生物医学期刊上发表，包括（过去5-6年）《科学》、《自然》、《细胞》、《自然遗传学》、《自然方法》、《神经元》、《细胞代谢》、《发育细胞》、PNAS（美国）、PLoS遗传学、基因与发育和发展。因此，我们寻求扩大的设施促进了尖端生物医学研究，并在支持美国大学的生物医学研究方面发挥了非常重要的作用。CZAR成立于2001年，通过20国集团资助机制，由美国大学和美国国立卫生研究院提供配套资金。它的设计是为了支持五位职业生涯早期到中期的研究者的研究项目。在一个大胆而创新的举措中，2002年，CZAR成为一个开放使用的共享核心设施，服务于整个U of U社区，每年从U获得运营支持和定期仪器资金。除了动物住房，护理和统一监督，作为核心设施，CZAR提供：1)实验程序的空间和设备；2)教学和专业知识；3)获得尖端技术；4)多个研究小组之间的合作机会。据我们所知，CZAR是美国第一个用于斑马鱼研究的大学核心设施。Core吸引了许多在uu的成熟研究人员开始研究斑马鱼，并成为我们为uu的健康科学中心招募许多初级斑马鱼研究人员的基础（在过去的四年中有五名新教员）。沙皇迫切需要扩张。目前有80多名各级研究人员使用Core，在过去的5年里，Core一直以90%以上的住房容量运行。新招募的教师，最近获得了美国国立卫生研究院“加强斑马鱼研究计划”（PAR-11-130和-131）和“主任新创新者奖励计划”（RFA-RM-12-016）的资助，以产生新的突变体和转基因系，供全球斑马鱼研究界使用，并且最近在U开发的基因敲除和基因靶向技术提供了新的研究机会，需要在CZAR增加住房和实验空间。目前的沙皇大厦面积为3646平方英尺；它包含70个6架60“机架（5040个3升的水箱），用于斑马鱼的标准住房，以及一个用于隔离动物的机架。拟议的1,129,380美元项目将使CZAR的容量扩大50%以上。我们向NIH申请50万美元；该项目的具体目标是：1)翻新1,923平方英尺的现有建筑空间，紧邻当前的CZAR，以扩大当前的CZAR；2)购买并安装36个新的6架60英寸机架，从而使CZAR的斑马鱼住房容量增加51%；3)将检疫能力提高一倍；4)建立新的实验程序区，包括一个非周期生产鸡蛋的房间。该项目涉及《FOA》的若干目标。它有效地满足了进行公共卫生系统资助的生物医学研究的实际需要。由于它是一个共享的核心设施，因此可以有效地利用该设施来适应个人研究项目不断变化的需求。通过在现有的CZAR核心设施范围内扩大斑马鱼的住房容量，该设施在全职，经验丰富的专业人员的监督下运作，该项目保证了对动物的最高护理质量，并严格遵守美国农业部动物福利法和国土安全部有关护理和使用实验动物的政策。最后，拟议的改造项目始终采用可持续设计技术和设计方法。