FIBRONECTIN RECEPTOR MEDIATED ADHESION OF P GINGIVALIS

大约75%的成年人患有某种形式的 牙周病 牙周病毒革时引起 阴性细菌定植在牙龈缝隙中。 这种殖民化 涉及细菌粘连和上皮细胞之间的特定相互作用， 细胞表面受体 在血清，唾液， 裂隙液也可参与这些粘连事件。 牙龈卟啉单胞菌是革兰氏阴性专性厌氧菌， 通常从牙周袋中分离。 其坚持口头 上皮是有据可查的，但这种相互作用的分子基础 不明 已知纤连蛋白参与许多重要的细胞凋亡。 涉及受体的整合素家族的粘附事件，以及许多 这些受体已在上皮细胞上被鉴定。 此外，本发明还提供了一种方法， 纤连蛋白可以以不溶性形式表达在细胞表面， 上皮细胞，也由它们分泌。 此外，可溶性纤连蛋白是 唾液和血清中都有 最后，几种细菌菌株 已经鉴定出能够结合各种形式的人纤连蛋白的人纤连蛋白。 因此，本建议的目的是确定纤维连接蛋白的作用， 牙龈卟啉单胞菌对口腔上皮细胞的粘附。 一旦角色 纤维连接蛋白在细菌粘附中的作用， 能够抑制这种最初的殖民活动。 因此，本提案的具体目标是：（1）确定 牙龈卟啉单胞菌与纤维连接蛋白之间的相互作用;（2）表征 口腔粘膜纤维连接蛋白表达和/或纤维连接蛋白结合能力 上皮细胞;和（3）确定纤维连接蛋白的潜在作用， 牙龈卟啉单胞菌与口腔上皮的结合。 牙龈卟啉单胞菌与可溶性的和/或可溶性的细菌相互作用的能力是显著的。 不溶性纤连蛋白将通过ELISA测定。 两者 上皮细胞系和新鲜分离的口腔上皮细胞将被 通过流式细胞术分析以确定细胞表面 纤连蛋白，纤连蛋白受体，以及受体的能力， 结合纤连蛋白。 将进行粘附试验，以确定 牙龈卟啉单胞菌和口腔上皮的结合特性。 重要的是 使用已建立的细胞系作为细菌体外模型的能力 还将研究口腔中的粘附。

Approximately 75% of the adult population suffers from some form of periodontal disease. Periodontal disease results when virulent gram negative bacteria colonize the gingival crevice. This colonization involves specific interactions between bacterial adhesions and epithelial cell surface receptors. Secreted molecules found in serum, saliva, and crevicular fluid can also participate in these adhesion events. Porphyromonas gingivalis is a gram negative obligate anaerobe that is commonly isolated from periodontal pockets. Its adherence to oral epithelium is well documented, but the molecular basis for this interaction is unknown. Fibronectin is known to participate in a number of important adhesion events involving the integrin family of receptors, and a number of these receptors have been identified on epithelial cells. In addition, fibronectin can be expressed in an insoluble form on the surface of epithelial cells and also secreted by them. Also, soluble fibronectin is found in both saliva and serum. Finally, several bacterial strains have been identified which are able to bind various forms of human fibronectin. Thus the objective of this proposal is to determine the role of fibronectin in the adhesion of P. gingivalis to oral epithelial cells. Once the role of fibronectin in bacterial adhesion is determined, preventive therapies could to be developed which would inhibit this initial colonization event. Accordingly, the specific aims of this proposal are: (1) to characterize the interactions between P. gingivalis and fibronectin; (2) to characterize the fibronectin expression and/or fibronectin binding capacity of oral epithelium; and (3) to determine the potential role of fibronectin in the binding of P. gingivalis to oral epithelium. The ability of P. gingivalis to interact with either soluble and/or insoluble fibronectin will be determined by means of an ELISA. Both an epithelial cell line and freshly isolated oral epithelial cells will be analyzed by flow cytometry to determine the presence of cell surface fibronectin, receptors for fibronectin, and the ability of the receptors to bind fibronectin. Adhesion assays will be performed to determine the binding properties of P. gingivalis and oral epithelium. Importantly, the ability to use an established cell line as an in vitro model for bacterial adhesion in the oral cavity will also be investigated.