HINDBRAIN INVOLVEMENT IN WATER AND SODIUM INTAKE

后脑参与水和钠的摄入

• 批准号: 2142351
• 负责人: GAYLEN L EDWARDS
• 金额: $ 10.21万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-15 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2142351
• 关键词: afferent nerve; angiotensin II; area postrema; atrium; baroreceptors; digital imaging; electrolyte balance; ethology; experimental brain lesion; fluorescence microscopy; immunocytochemistry; isoproterenol; laboratory rat; neural information processing; neuroanatomy; neuropharmacology; nutrition related tag; parabrachial nucleus; salt intake; serotonin; serotonin receptor; sodium; solitary tract nucleus; stereotaxic techniques; stimulant /agonist; taste; thirst regulatory center; water drinking behavior

Remarkable alterations in the regulation of water and saline intake have been noted in animals with lesions in the dorsal hindbrain. More recently, studies indicate that lesions placed in the area postrema/adjacent nucleus of the solitary tract result in greatly increased consumption of water and saline. Moreover, very similar changes in water intake are noted after lesions that include the lateral parabrachial nucleus, a pontine nucleus that receives direct input from the area postrema and nucleus of the solitary tract. These observations suggest that the area postrema and nucleus of the solitary tract in the medulla and the lateral parabrachial nucleus in the pons are components of an ascending inhibitory system that acts to inhibit the consumption of fluids to replenish the extracellular fluid compartment. The nature of inhibitory signals in this pathway or the precise site(s) at which they might act have yet to be elucidated. Thus, a careful examination of the dorsomedial hindbrain and its projection sites is necessary to develop our understanding of the mechanisms involved in the control of water and electrolyte balance. The studies presented here will examine several aspects of this proposed inhibitory hindbrain pathway. Experiment 1 will examine the specific role of the nucleus of the solitary tract in this inhibitory pathway. Experiment 2 will examine the functional interaction between the area postrema, nucleus of the solitary tract and lateral parabrachial nucleus in this pathway. Experiment 3 will examine the pharmacology of the projections from the area postrema/nucleus of the solitary tract to the lateral parabrachial nucleus. Finally, experiment 4 will study the possibility that information from atrial receptors is disrupted by these lesions. The present research plan will examine the proposed hindbrain inhibitory pathway using a variety of methods including behavioral, physiological, pharmacological and anatomical analyses. Since many of these methods, such as 3 dimensional image analysis, have only recently become available, these studies will provide fundamental new information about the interaction of nuclei in the hindbrain. These systems can be dissected anatomically, functionally and pharmacologically. The analysis of these hindbrain nuclei will aid in elucidating the physiology and neurobiology underlying this hindbrain system and extend our knowledge of neural regulation of fluid and electrolyte balance.

水和盐水摄入量的调节发生了显著的变化 在背侧后脑受损的动物中已被发现。最近， 研究表明，位于最后/邻近核的病变 会导致大量的用水量增加， 生理盐水。此外，水摄入量的变化也非常相似。 病变包括臂旁外侧核、桥核 它接受来自最后区和丘脑核团的直接信息输入 孤立区。这些观察结果表明，最后区域和 延髓孤束核和臂旁外侧核 脑桥中的核是上升抑制系统的组成部分 作用于抑制液体的消耗以补充细胞外 液体舱。这条通路中抑制性信号的性质或 他们可能在哪里(S)采取行动的确切地点尚未阐明。因此，一个 仔细检查背内侧后脑及其投射部位 是必要的，以加深我们对 水和电解质平衡的控制。 这里提出的研究将检验这一提议的几个方面 抑制后脑通路。实验1将考察特定的角色 在这条抑制通路中的孤束核。 实验2将考察该区域之间的功能相互作用。 最后核、孤束核和臂旁外侧核 这条路。实验3将考察黄连的药理作用。 最后区/孤束核至孤束核的投射 臂旁外侧核。最后，实验4将研究 来自心房感受器的信息可能被这些 损伤。 目前的研究计划将检验拟议的后脑抑制。 使用各种方法，包括行为、生理、 药理和解剖学分析。由于这些方法中的许多方法，如 作为三维图像分析，最近才出现，这些 研究将提供基本的新信息，关于 后脑中的核。这些系统可以被解剖解剖， 在功能上和药理上。这些后脑核团的分析 将有助于阐明其背后的生理学和神经生物学 后脑系统，并扩展我们对液体和神经调节的知识 电解质平衡。