HINDBRAIN INVOLVEMENT IN WATER AND SODIUM INTAKE

后脑参与水和钠的摄入

• 批准号: 3464141
• 负责人: GAYLEN L EDWARDS
• 金额: $ 9.31万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-15 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3464141
• 关键词: afferent nerve; angiotensin II; area postrema; atrium; baroreceptors; digital imaging; electrolyte balance; ethology; experimental brain lesion; fluorescence microscopy; immunocytochemistry; isoproterenol; laboratory rat; neural information processing; neuroanatomy; neuropharmacology; nutrition related tag; parabrachial nucleus; salt intake; serotonin; serotonin receptor; sodium; solitary tract nucleus; stereotaxic techniques; stimulant /agonist; taste; thirst regulatory center; water drinking behavior

Remarkable alterations in the regulation of water and saline intake have been noted in animals with lesions in the dorsal hindbrain. More recently, studies indicate that lesions placed in the area postrema/adjacent nucleus of the solitary tract result in greatly increased consumption of water and saline. Moreover, very similar changes in water intake are noted after lesions that include the lateral parabrachial nucleus, a pontine nucleus that receives direct input from the area postrema and nucleus of the solitary tract. These observations suggest that the area postrema and nucleus of the solitary tract in the medulla and the lateral parabrachial nucleus in the pons are components of an ascending inhibitory system that acts to inhibit the consumption of fluids to replenish the extracellular fluid compartment. The nature of inhibitory signals in this pathway or the precise site(s) at which they might act have yet to be elucidated. Thus, a careful examination of the dorsomedial hindbrain and its projection sites is necessary to develop our understanding of the mechanisms involved in the control of water and electrolyte balance. The studies presented here will examine several aspects of this proposed inhibitory hindbrain pathway. Experiment 1 will examine the specific role of the nucleus of the solitary tract in this inhibitory pathway. Experiment 2 will examine the functional interaction between the area postrema, nucleus of the solitary tract and lateral parabrachial nucleus in this pathway. Experiment 3 will examine the pharmacology of the projections from the area postrema/nucleus of the solitary tract to the lateral parabrachial nucleus. Finally, experiment 4 will study the possibility that information from atrial receptors is disrupted by these lesions. The present research plan will examine the proposed hindbrain inhibitory pathway using a variety of methods including behavioral, physiological, pharmacological and anatomical analyses. Since many of these methods, such as 3 dimensional image analysis, have only recently become available, these studies will provide fundamental new information about the interaction of nuclei in the hindbrain. These systems can be dissected anatomically, functionally and pharmacologically. The analysis of these hindbrain nuclei will aid in elucidating the physiology and neurobiology underlying this hindbrain system and extend our knowledge of neural regulation of fluid and electrolyte balance.

水和盐摄入调节的显著变化 在后脑背侧有病变的动物中观察到。 最近， 研究表明位于最后区/邻近核的病变 孤立道的形成导致水的消耗大大增加， 盐水 此外，非常相似的变化，在水的摄入量注意到后， 包括臂旁外侧核、脑桥核 接受来自最后区和核团的直接输入， 孤束 这些观察结果表明，最后区和 延髓孤束核和臂旁外侧核 脑桥中的核是上行抑制系统的组成部分， 作用是抑制液体的消耗，以补充细胞外 液体室 在这一途径中抑制信号的性质或 它们可能发生作用的确切地点尚待阐明。 因此 仔细检查后脑背内侧及其投射部位 是必要的，以发展我们的理解的机制， 控制水和电解质平衡。 这里提出的研究将审查这一建议的几个方面 抑制性后脑通路 实验1将考察 孤束核在抑制通路中的作用。 实验2将考察脑区与脑区之间的功能相互作用 后束核、孤束核和臂旁外侧核 这条路。 实验3将检查 从孤束最后区/孤束核到 臂旁外侧核 最后，实验4将研究 来自心房感受器的信息被这些干扰的可能性 病变 目前的研究计划将检查拟议的后脑抑制 途径使用各种方法，包括行为，生理， 药理学和解剖学分析。 由于这些方法中的许多方法，例如 作为三维图像分析，只是最近才变得可用， 研究将提供关于以下因素相互作用的基本新信息： 后脑的核团。 这些系统可以从解剖学上解剖， 功能上和功能上。 对这些后脑核团的分析 将有助于阐明这背后的生理学和神经生物学 后脑系统和扩展我们的知识神经调节的流体和 电解质平衡