VESTIBULAR REGULATION OF RESPIRATORY MUSCLE ACTIVITY

呼吸肌活动的前庭调节

• 批准号: 2128107
• 负责人: ALAN D MILLER
• 金额: $ 25.83万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2128107
• 关键词: brain stem; cats; decerebration; electrostimulus; experimental brain lesion; head movements; laryngeal nerves; medulla oblongata; neuronal transport; posture; pulmonary respiration; respiratory muscles; vagus nerve; vestibular apparatus; vestibular nerve; vestibular nuclei

The vestibular system produces a number of compensatory responses to accelerations imposed upon the head. Probably the least understood of these are vestibular influences on thoracic and abdominal "respiratory" muscles which also have postural and cardiovascular functions. The goal of this project is to determine both the functional significance of vestibular-evoked respiratory muscle responses and the neural substrate that produces these responses. Experiments will be conducted using the cat, for which there is considerable relevant background information concerning the vestibular and respiratory systems. Two types of experiments will be performed to gain insight into the functional significance of vestibular-respiratory responses, using the decerebrate cat preparation. First, the extent of the responses will be examined. In particular, we will determine if the activity of the muscles of the upper airway, like that of the respiratory muscles of the thorax and abdomen, is influenced by electrical stimulation of the vestibular nerve. Second, natural vestibular stimulation in multiple vertical planes and in the horizontal plane will be used to determine the directional sensitivity and dynamics of the respiratory muscle response. The neural substrate that produces the vestibulo-respiratory response will be examined using three types of experiments. First, the anterograde neuroanatomical tracer Phaseolus vulgaris leucoagglutinin (PHA-L) will be iontophoretically injected into the medial and adjacent inferior vestibular nuclei to determine which areas containing respiratory neurons may receive direct vestibular inputs. The medial and inferior nuclei were chosen for study since they are known to be essential for mediating vestibulo-respiratory responses. Second, the responses of medullary respiratory neurons to electrical and natural vestibular input will be determined. Our preliminary data indicate that at least some of these neurons are responsive to vestibular stimulation. Finally, medullary respiratory groups will be inactivated using chinook acid microinjections to determine their importance in mediating vestibulo-respiratory responses.

前庭系统会产生多种补偿反应 施加在头部的加速度。 可能是最不被理解的 这些是前庭对胸部和腹部“呼吸”的影响 肌肉也具有姿势和心血管功能。 目标 这个项目的目的是确定两者的功能意义 前庭诱发的呼吸肌反应和神经基质 产生这些反应。 实验将使用 cat，有大量相关背景信息 关于前庭和呼吸系统。 将进行两种类型的实验来深入了解 前庭呼吸反应的功能意义，使用 猫去脑准备。 首先，响应的范围将是 检查了。 特别是，我们将确定肌肉的活动是否 上呼吸道，如胸部呼吸肌 和腹部，受到前庭电刺激的影响 神经。 二、多个垂直平面的自然前庭刺激 并在水平面上将用于确定方向 呼吸肌反应的敏感性和动力学。 产生前庭呼吸反应的神经基质将 使用三种类型的实验进行检查。 一、顺行 神经解剖学示踪剂菜豆白细胞凝集素 (PHA-L) 将 离子电渗疗法注射到内侧和相邻下侧 前庭核以确定哪些区域包含呼吸神经元 可以接收直接的前庭输入。 内侧核和下核是 选择进行研究是因为众所周知它们对于调解至关重要 前庭呼吸反应。 二、髓质反应 呼吸神经元对电和自然前庭输入的影响 决定。 我们的初步数据表明，至少其中一些 神经元对前庭刺激有反应。 最后是髓质 使用奇努克酸显微注射可灭活呼吸群 确定它们在调节前庭呼吸中的重要性 回应。