CHRONIC FLUORIDE EFFECTS ON BONE STRENGTH IN RATS

慢性氟化物对大鼠骨强度的影响

• 批准号: 2132522
• 负责人: CHARLES H TURNER
• 金额: $ 9.11万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2132522
• 关键词: X ray crystallography; aging; bone; bone density; bone fracture; densitometry; diabetes mellitus; dietary calcium; elasticity; femur; fluorine; laboratory rat; normal ossification; nutrition related tag; renal failure; scanning electron microscopy; sound; spine; tensile strength

DESCRIPTION: Recent epidemiological evidence suggests higher fracture incidence in communities with fluoridated water. It is conceivable that an increase in fractures may be due to a decrease in bone strength caused by chronic fluoride exposure. Pilot data from rats shows that fluoride reduces bone strength more dramatically in older animals. Thus, bone fluoride accumulation may lead to increased fracture risk in older animals. There is also evidence that fluoride incorporation into the skeleton is altered by dietary changes, especially changes in calcium intake.In this application, the Principal Investigator proposes to measure mechanical and material properties of fluorotic bone from femurs and vertebrae of normal and physiologically challenged rats. Bone specimens will be obtained from Dr. Ann Dunipace of the Oral Health Research Institute at Indiana University, who is Principal Investigator of a grant entitled "Effects of fluoride in medically compromised animals". The Principal Investigator proposes to work in collaboration with Dr. Dunipace and test bone specimens from rats maintained under conditions of aging, diabetes, chronic renal failure and nutritional deficiencies. The hypothesis to be tested is that fluoride accumulation in the bone mineral leads to progressively decreased mechanical properties. It is expected that age and diet will alter the severity of the fluoride effect on bone strength. Bones from diabetic rats or rats with chronic renal failure are expected to accumulate more fluoride in their skeletons,resulting in greater bone strength deficit. It is also hypothesized that fluoride ion distribution in the bone mineral will have a direct effect on the mineralization and elasticity of the bone matrix.

产品说明： 最近的流行病学证据表明 更高 饮用含氟水的社区骨折发生率。是 可以想象，骨折的增加可能是由于 对骨骼强度的影响 导频数据 研究表明氟化物降低骨骼强度的作用 在年长的动物身上发生了巨大的变化。因此，骨氟蓄积可能 导致老年动物骨折风险增加。 还有 有证据表明，氟化物进入骨骼的过程被改变， 饮食变化，特别是钙摄入量的变化。在这方面， 应用程序，主要研究者建议测量 股骨氟骨的力学和材料特性 和正常和生理学挑战的大鼠的椎骨。 骨 样本将从口腔健康中心的Ann Dunipace博士处获得 印第安纳州大学研究所，首席研究员 一项名为“氟化物在医疗损害中的影响”的赠款， 动物”。首席研究员建议在 与Dunipace博士合作，测试大鼠骨骼标本 在衰老、糖尿病、慢性肾衰竭的条件下维持 和营养不良。 有待检验的假设是， 氟在骨矿物质中的积累导致 机械性能下降。预计年龄和饮食将 改变氟化物对骨强度影响的严重程度。 骨头 来自糖尿病大鼠或患有慢性肾衰竭的大鼠， 在骨骼中积累更多的氟化物， 力量不足。 还假设氟离子 分布 骨矿物质会直接 效果 骨基质的矿化和弹性。