PERIODONTAL DISEASE IN TWINS

双胞胎牙周病

• 批准号: 2129969
• 负责人: BRYAN S MICHALOWICZ
• 金额: $ 11.06万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2129969
• 关键词: Actinobacillus actinomycetemcomitans; Bacteroides gingivalis; Fusobacterium nucleatum; dental disorder diagnosis; dental plaque; dental radiography; dental survey; dizygotic twins; family genetics; gram negative bacteria; human middle age (35-64); human subject; monoclonal antibody; monozygotic twins; oral health; periodontitis

The long-term goal of this project is to determine the influence of host genetic and environmental factors on clinical, radiographic, and microbiological measures of periodontal disease through the study of twins. Periodontal disease affects three-fourths of American adults, and it is estimated that five to six billion dollars per year is required to treat existing disease. Although clinical and bacterial risk factors have been well-studied, the influence that host genetic factors have on this disease is largely undetermined. In years 01-03, we established a Minnesota Periodontal Twin Registry which includes 175 pairs of adult monozygous and dizygous twins reared together and apart. Clinical periodontal measures, dental radiographs, and subgingival plaque samples were obtained from all consenting subjects. Analyses to date indicate that a significant portion of the population variance for clinical and radiographic measures of disease are attributable to genetic variance. The specific aims of this current proposal are to: 1) recruit and evaluate 36 additional pairs of older (equal to or greater than 50 years of age) reared-together twins to increase our sample of twins with moderate to severe periodontal disease; 2) evaluate 24 additional pairs of reared-apart twins; 3) assay archived and subsequently obtained plaque samples from 235 pairs of twins using monoclonal antibodies specific against five putative periodontal microbial pathogens; and 4) determine the influence of genetic, early family environmental, and unshared environmental factors on the clinical, radiographic, and microbiological measures of periodontal disease. Volunteers will be recruited through the Minnesota Center for Twin and Adoption Research. Each twin will be independently evaluated by two calibrated examiners who will be blinded to the subject's zygosity. Clinical measures of disease will be recorded and subgingival plaque samples obtained from six index teeth and in one randomly selected quadrant. Both univariate and multivariate data analyses will be completed, and several twin models will be tested to determine the most appropriate model of the genetic and environmental components of phenotypic variance. Determining the influence of genetic factors in periodontal disease may lead to the development of early and aggressive preventive strategies for those individuals most at risk for developing disease or acquiring pathogenic oral microorganisms.

该项目的长期目标是确定 宿主遗传和环境因素对临床、放射学和 牙周病的微生物学措施，通过研究 双胞胎 牙周病影响四分之三的美国成年人， 据估计，每年有50亿到60亿美元， 需要治疗现有疾病。 虽然临床和细菌 风险因素已得到充分研究，宿主遗传因素的影响 对这种疾病的影响在很大程度上是不确定的。 在01-03年，我们建立了明尼苏达期刊双胞胎登记处 其中包括175对成年的单卵和双卵双胞胎， 一起和分开。 临床牙周测量，牙科X光片， 和龈下菌斑样本从所有同意 科目 迄今为止的分析表明， 疾病的临床和放射学测量的群体方差 是由遗传变异引起的。 具体目标是 目前的建议是：1）招募和评估36个额外的对 年龄较大（等于或大于50岁） 双胞胎，以增加我们的双胞胎样本， 牙周病; 2）评估24对额外的分离 双胞胎; 3）试验存档，随后从 235对双胞胎使用单克隆抗体， 假定的牙周微生物病原体;和4）确定 遗传、早期家庭环境和非共享影响 环境因素对临床、影像学和 牙周病的微生物学指标。 志愿者将通过明尼苏达州双胞胎中心招募， 收养研究。 每对双胞胎将由两个独立的评估 对受试者的接合性不知情的校准检查员。 将记录疾病的临床指标，并记录龈下菌斑 从六颗索引牙齿和一颗随机选择的牙齿中获得样本 象限。 单变量和多变量数据分析都将 完成，并将测试几个双胞胎模型，以确定最 遗传和环境因素的适当模型 表型方差 确定遗传因素的影响 牙周病可能会导致早期和积极的发展 为那些最有可能发展成 疾病或获得病原性口腔微生物。