PERIODONTAL DISEASE IN TWINS

该项目的长期目标是确定 宿主遗传和环境因素对临床、放射学和 牙周病的微生物学研究进展 双胞胎。牙周病影响着四分之三的美国成年人， 据估计，每年50亿至60亿美元 治疗现有疾病所需的。尽管临床和细菌 风险因素已经得到了很好的研究，宿主遗传的影响 影响这种疾病的因素在很大程度上是不确定的。 在01-03年度，我们成立了明尼苏达州牙周双胞胎登记处 其中包括175对成年同卵双胞胎和异卵双胞胎 无论在一起还是分开。临床牙周检查，牙科X光片， 从所有同意的人那里获取了牙周下菌斑样本 研究对象。到目前为止的分析表明，很大一部分 疾病的临床和放射测量的总体差异 可归因于遗传变异。这样做的具体目的是 目前的建议是：1)招募和评估另外36对 指一起抚养的年龄较大(等于或大于50岁)的 双胞胎将增加我们的双胞胎样本，从中度到重度 牙周病；2)评估另外24对分开抚养的人 双胞胎；3)化验存档并随后从 235对双胞胎使用了5对特异性的单抗 可能的牙周微生物病原体；以及4)确定 遗传、早期家庭环境和非共同的影响 环境因素对临床、X线片和 牙周病的微生物学检测。 志愿者将通过明尼苏达州双胞胎中心招募 领养研究。每对双胞胎将由两个人独立评估 经过校准的考官，他们对受试者的合格性视而不见。 将记录疾病的临床措施和龈下菌斑 样本取自6颗索引牙齿和随机选择的1颗 象限。单变量和多变量数据分析都将 建成后，将对几个双胞胎模型进行测试，以确定最 遗传和环境成分的适当模型 表型变异。确定遗传因素对遗传因素的影响 牙周病可能会导致发展的早期和侵袭性 为那些最有可能罹患癌症的人制定预防策略 疾病或获得致病的口腔微生物。

The long-term goal of this project is to determine the influence of host genetic and environmental factors on clinical, radiographic, and microbiological measures of periodontal disease through the study of twins. Periodontal disease affects three-fourths of American adults, and it is estimated that five to six billion dollars per year is required to treat existing disease. Although clinical and bacterial risk factors have been well-studied, the influence that host genetic factors have on this disease is largely undetermined. In years 01-03, we established a Minnesota Periodontal Twin Registry which includes 175 pairs of adult monozygous and dizygous twins reared together and apart. Clinical periodontal measures, dental radiographs, and subgingival plaque samples were obtained from all consenting subjects. Analyses to date indicate that a significant portion of the population variance for clinical and radiographic measures of disease are attributable to genetic variance. The specific aims of this current proposal are to: 1) recruit and evaluate 36 additional pairs of older (equal to or greater than 50 years of age) reared-together twins to increase our sample of twins with moderate to severe periodontal disease; 2) evaluate 24 additional pairs of reared-apart twins; 3) assay archived and subsequently obtained plaque samples from 235 pairs of twins using monoclonal antibodies specific against five putative periodontal microbial pathogens; and 4) determine the influence of genetic, early family environmental, and unshared environmental factors on the clinical, radiographic, and microbiological measures of periodontal disease. Volunteers will be recruited through the Minnesota Center for Twin and Adoption Research. Each twin will be independently evaluated by two calibrated examiners who will be blinded to the subject's zygosity. Clinical measures of disease will be recorded and subgingival plaque samples obtained from six index teeth and in one randomly selected quadrant. Both univariate and multivariate data analyses will be completed, and several twin models will be tested to determine the most appropriate model of the genetic and environmental components of phenotypic variance. Determining the influence of genetic factors in periodontal disease may lead to the development of early and aggressive preventive strategies for those individuals most at risk for developing disease or acquiring pathogenic oral microorganisms.