MECHANISMS OF OROFACIAL NOCICEPTION AND THERMORECEPTION

产品说明： 本项目的总体目标是阐明 温度感受和伤害感受的中枢机制。的 拟议项目的具体目标是确定 三叉神经脑干温度感受神经元的特性 复合体和丘脑及其投射靶点，以及 检查热感受传入输入的相互作用， 伤害感受神经元的反应。 细胞外电生理 将在麻醉大鼠中采用单单位记录技术， 猫记录将集中在位于边缘的神经元上 髓背角（MDH）层，尽管可能存在 在MDH的深层和 还将检查三叉神经极间亚核。的答复 温感受神经元对无害和有害的冷热刺激的反应 将被定性。这些神经元的投射靶点在 丘脑将通过逆行标测来确定 技术. 皮层下行影响的影响， 脑干将被确定。 其他实验将检查 皮肤降温对大鼠海马伤害性神经元反应的影响 MDH。在第二部分的项目录音将在 猫和大鼠的丘脑和丘脑的反应和位置 热感受神经元的特点。这些实验将提供 三叉神经温度信息处理的新数据 脊髓束核和丘脑，并阐明其机制 冷痛。他们还将研究热的相互作用， 皮肤输入与伤害性输入，并可能提供新的见解 伤害性信息的中枢处理

DESCRIPTION: The general goals of this project are to elucidate the central mechanisms underlying thermoreception and nociception. The specific aims of the proposed project are to determine the response properties of thermoreceptive neurons in the trigeminal brainstem complex and thalamus and their projection targets as well as to examine the interaction of thermoreceptive afferent inputs on the responses of nociceptive neurons. Electrophysiological extracellular single unitrecording techniques will be employed in anesthetized rats and cats. Recordings will concentrate on neurons located in the marginal layer of the medullary dorsal horn (MDH) although the possible existence of thermoreceptive neurons in deeper layers of the MDH and in trigeminal subnucleus interpolaris will also be examined. The responses of thermoreceptive neurons to innocuous and noxious cold and hot stimuli will be characterized. The projection targets of these neurons in thalamus will be determined by means of antidromic mapping techniques. The influence of descending influences from cortex and brainstem will be ascertained. Other experiments will examined the effects of skin cooling on the responses of nociceptive neurons in the MDH. In the second part of the project recordings will be made in thalamus of cats and rats and the responses and locations of thalamic thermoreceptive neurons characterized. These experiments will provide new data on the processing of temperature information in the trigeminal spinal tract nucleus and thalamus and clarify the mechanisms underlying cold pain. They will also examine the interactions of thermal cutaneous inputs with nociceptive inputs and may provide new insights on the central processing of nociceptive information.