CHRONIC FLUORIDE EFFECTS ON BONE STRENGTH IN RATS

慢性氟化物对大鼠骨强度的影响

• 批准号: 2132523
• 负责人: CHARLES H TURNER
• 金额: $ 8.81万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2132523
• 关键词: X ray crystallography; aging; bone; bone density; bone fracture; densitometry; diabetes mellitus; dietary calcium; elasticity; femur; fluorine; laboratory rat; normal ossification; nutrition related tag; renal failure; scanning electron microscopy; sound; spine; tensile strength

DESCRIPTION: Recent epidemiological evidence suggests higher fracture incidence in communities with fluoridated water. It is conceivable that an increase in fractures may be due to a decrease in bone strength caused by chronic fluoride exposure. Pilot data from rats shows that fluoride reduces bone strength more dramatically in older animals. Thus, bone fluoride accumulation may lead to increased fracture risk in older animals. There is also evidence that fluoride incorporation into the skeleton is altered by dietary changes, especially changes in calcium intake.In this application, the Principal Investigator proposes to measure mechanical and material properties of fluorotic bone from femurs and vertebrae of normal and physiologically challenged rats. Bone specimens will be obtained from Dr. Ann Dunipace of the Oral Health Research Institute at Indiana University, who is Principal Investigator of a grant entitled "Effects of fluoride in medically compromised animals". The Principal Investigator proposes to work in collaboration with Dr. Dunipace and test bone specimens from rats maintained under conditions of aging, diabetes, chronic renal failure and nutritional deficiencies. The hypothesis to be tested is that fluoride accumulation in the bone mineral leads to progressively decreased mechanical properties. It is expected that age and diet will alter the severity of the fluoride effect on bone strength. Bones from diabetic rats or rats with chronic renal failure are expected to accumulate more fluoride in their skeletons,resulting in greater bone strength deficit. It is also hypothesized that fluoride ion distribution in the bone mineral will have a direct effect on the mineralization and elasticity of the bone matrix.

描述：最近的流行病学证据表明 含氟水社区的骨折发生率。它是 可以想象，骨折的增加可能是由于 慢性氟化物暴露导致的骨骼强度下降。试点数据 来自大鼠的研究表明，氟化物更能降低骨骼强度 在年长的动物身上戏剧性地表现出来。因此，骨氟蓄积可能 导致老年动物骨折风险增加。还有就是 氟化物进入骨骼的证据是由 饮食的变化，尤其是钙摄入量的变化。 申请，首席调查员建议衡量 股骨氟化骨的力学和材料特性 以及正常大鼠和生理挑战大鼠的椎骨。骨 样本将从口腔健康的Ann Dunipace博士处获得 印第安纳大学研究院，首席研究员 一项名为“氟化物对医学危害的影响”的拨款 动物。首席调查员建议在 与Dunipace博士合作并测试大鼠的骨标本 在衰老、糖尿病、慢性肾功能衰竭的情况下维持 和营养缺乏。需要检验的假设是 氟化物在骨矿物质中的积累导致进行性 机械性能下降。预计年龄和饮食将 改变氟化物对骨骼强度影响的严重程度。骨头 糖尿病大鼠或慢性肾功能衰竭大鼠的 在他们的骨骼中积累更多的氟，导致更大的骨骼 力量不足。它还假设氟离子 在骨矿中的分布会有直接的影响 关于骨基质的矿化和弹性。