INTRACELLULAR PROCESSING OF RAT KIDNEY RENIN

大鼠肾肾素的细胞内加工

• 批准号: 2133720
• 负责人: LAURA L NORLING
• 金额: $ 7.28万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2133720
• 关键词: arterioles; endocrinology; enzyme activity; glycosylation; intracellular transport; kidney cell; kidney function; laboratory rabbit; laboratory rat; protein isoforms; renin; secretion; tissue /cell culture; vasomotion; western blottings

The physiologic importance of the renin-angiotensin system (RAS) in maintaining cardiovascular homeostasis is undisputed. Recent observations have expanded the traditional views of the morphology and function of renin-secreting cells. A) Two distinct renal cell types, epithelioid juxtaglomerular cells (JG cells) and distal afferent arteriolar smooth muscle cells, are known to contain and secrete renin. B) The renin angiotensin system has local and systemic actions in controlling cardiovascular homeostasis. C) Renin isoforms with differing metabolic half lives have been identified. The working hypothesis is that JG cells and distal afferent arteriolar cells contain and secrete different renin isoforms. Through these differences the two cell types may contribute in distinct ways to regulation of vascular homeostasis. The long term goal of this proposal is to define the secretory patterns and renin isoforms of unstimulated and stimulated renin release in rat kidney JG cells and distal afferent arteriolar cells. In this proposal, Dr. Norling will apply cell biology techniques of pulse chase analysis of secretion for studying renin secretion in two renin- containing cell types from rat kidney. Renin isoforms from these cells will be characterized and compared. From these experiments it is expected that Dr. Norling will find that JG cells and distal afferent arteriolar cells have renin processing capabilities which are cell-type specific. This information will help to expand the understanding of RAS regulation of vascular tone.

肾素-血管紧张素系统(RAS)的生理学意义 维持心血管内环境平衡是毋庸置疑的。近期 观察扩展了传统的形态学观点和 肾素分泌细胞的功能。A)两种不同的肾细胞类型， 上皮样球旁细胞(JG细胞)和远端传入 小动脉平滑肌细胞，已知含有和分泌肾素。 B)肾素血管紧张素系统在 控制心血管动态平衡。C)不同的肾素异构体 新陈代谢半衰期已经确定。工作假说是 JG细胞和远端传入小动脉细胞含有和分泌 不同的肾素亚型。通过这些差异，这两种细胞类型 可能以不同的方式对调节血管内稳态做出贡献。 这项提议的长期目标是定义分泌模式 和大鼠未刺激和刺激的肾素释放的肾素亚型 肾JG细胞和远端传入小动脉细胞。 在这项提案中，诺林博士将应用PULSE的细胞生物学技术 Chase分析用于研究两种肾素分泌的肾素 含有来自大鼠肾脏的细胞类型。来自这些细胞的肾素亚型 将被描述和比较。从这些实验来看， 预计诺林博士会发现JG细胞和远端传入 小动脉细胞具有肾素处理能力，这是细胞型的 具体的。这些信息将有助于扩大对RAS的理解 调节血管张力。