STRETCH, ION CHANNELS, & CARDIAC CELL VOLUME REGULATION

拉伸、离子通道、

• 批准号: 2210589
• 负责人: SIMON H CLEMO
• 金额: $ 8.52万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2210589
• 关键词: cell morphology; colchicine; cyclic nucleoside monophosphate; cytochalasins; cytoskeleton; heart cell; heart ventricle; intracellular transport; laboratory rabbit; membrane channels; microelectrodes; potassium channel; sodium channel; stretch receptors; vinblastine; voltage /patch clamp; voltage gated channel

The proposed research focuses on cardiac cell volume regulation, and studies will be carried out on freshly isolated single rabbit ventricular cells. Cardiac cell edema is well recognized in the setting of ischemia and congestive heart failure. Defects in cell volume regulation are associated with cellular damage as well as contractile and electrical dysfunction. Preliminary studies suggest that stretch-activated (SA) cation and anion channels may contribute to cardiac cell volume regulation. The candidate will utilize the patch clamp and ion selective microelectrode techniques to characterize the properties and regulation of SA cation and anion channels at the single channel level, and their contribution to membrane currents and intracellular ion activities of K+, Na+, and C1-. The combination of these electrophysiological measurements and cell volume determinations will provide new information about basic mechanisms of cell volume regulation. Further, the hypothesis that cytoskeletal elements and cyclic nucleotides modulate SA channels in heart will be tested. A better understanding of basic mechanisms of cell volume regulation is a precursor to developing strategies to minimize cellular damage during ischemia and congestive failure.

拟议的研究重点是心脏细胞体积调节， 研究将在新鲜分离的单个兔心室上进行 细胞 心肌细胞水肿是公认的在设置缺血 和充血性心力衰竭 细胞体积调节的缺陷是 与细胞损伤以及收缩和电 功能障碍 初步研究表明，牵张激活（SA） 阳离子和阴离子通道可能有助于心脏细胞体积 调控 候选人将利用膜片钳和离子选择性 微电极技术来表征性质和调节 SA阳离子和阴离子通道的单通道水平， 对膜电流和细胞内K+离子活性的贡献， Na+和C1-。 这些电生理测量的组合 细胞体积的测定将提供关于细胞的基本 细胞体积调节机制。 此外，假设 细胞骨架元件和环核苷酸调节SA通道， 心将受到考验。 更好地理解细胞的基本机制 音量调节是制定策略的先导， 缺血和充血性衰竭期间的细胞损伤。