CA++ SENSING IN A CALCITONIN SECRETING CELL LINE

降钙素分泌细胞系中的 CA 传感

• 批准号: 2133680
• 负责人: VERA T FAJTOVA
• 金额: $ 9.23万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2133680
• 关键词: G protein; biological signal transduction; calcitonin; calcium; calcium channel; calcium flux; calcium indicator; divalent cations; fluorimetry; inositol phosphates; secretion; tissue /cell culture

The goal of this proposal is to elucidate the mechanism of Ca2+ sensing in a C-cell line that is responsive to physiologic changes in extracellular Ca2+, rMTC 44-2. This cell line serves as a good model of Ca2+ sensing, particularly applicable to endocrine tissues of the Ca2+ homeostatic system. The specificity of the Ca2+-sensing apparatus will be examined using polyvalent cations other than Ca2+. The intracellular messengers involved in the transduction of the external Ca2+ signal will be examined. Single cell spectrofluorimetry will be used to study the cytosolic Ca2+ responses to stimulation. Polyphosphoinositide and cyclic nucleotide systems will be examined, and G-protein involvement will also be evaluated. The Endocrinology Division at the Brigham and Women's Hospital consists of a unique combination of faculty, comprising both clinical and basic science investigators, and physical resources. Investigators here have a broad spectrum of expertise, including electrophysiology of endocrine tissues, single cell spectrofluorimetry, receptor physiology, molecular biology and cell biochemistry. Clinical investigators include experts in Ca2+ homeostasis and osteoporosis, hypertension, neuroendocrinology and circadian biology. This diverse group meets for several weekly research and clinical conferences. The members of this department have at their disposal several fully equipped modern laboratories and an administrative core. The resources of the Harvard Medical School and Brigham and Women Hospital are available, including the opportunity to participate in graduate school courses and consult with members of other departments.

本研究的目的是阐明细胞内Ca ~（2+）敏感的机制， 一种对细胞外基质的生理变化有反应的C细胞系， Ca2+，rMTC 44-2。 该细胞系作为Ca 2+感应的良好模型， 特别适用于Ca 2+稳态的内分泌组织 系统 将检查Ca 2+感应装置的特异性 使用除Ca 2+以外的多价阳离子。 细胞内信使 将检查参与外部Ca 2+信号转导的细胞。 单细胞荧光分光光度法将用于研究细胞内Ca 2 + 对刺激的反应。 聚磷酸肌醇和环核苷酸 系统将进行检查，G蛋白参与也将进行评估。 布里格姆妇女医院的内分泌科包括： 独特的教师组合，包括临床和基础科学 调查人员和物质资源。 这里的调查人员有一个广泛的 专业知识，包括内分泌组织的电生理学， 单细胞荧光光谱法，受体生理学，分子生物学和 细胞生物化学 临床研究人员包括Ca 2+专家 稳态和骨质疏松症，高血压，神经内分泌学和 昼夜节律生物学 这个多元化的团体每周举行几次会议， 临床会议。 这个部门的成员在他们的 处置几个设备齐全的现代化实验室和一个行政 核心 哈佛医学院和布里格姆妇女组织的资源 医院提供，包括有机会参加 研究生院的课程，并与其他部门的成员进行磋商。