CA+2 SENSING IN A CALCITONIN SECRETING CELL LINE

降钙素分泌细胞系中的 CA 2 传感

• 批准号: 3080959
• 负责人: VERA T FAJTOVA
• 金额: $ 9.34万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080959
• 关键词: G protein; biological signal transduction; calcitonin; calcium; calcium channel; calcium flux; calcium indicator; divalent cations; fluorimetry; inositol phosphates; secretion; tissue /cell culture

The goal of this proposal is to elucidate the mechanism of Ca2+ sensing in a C-cell line that is responsive to physiologic changes in extracellular Ca2+, rMTC 44-2. This cell line serves as a good model of Ca2+ sensing, particularly applicable to endocrine tissues of the Ca2+ homeostatic system. The specificity of the Ca2+-sensing apparatus will be examined using polyvalent cations other than Ca2+. The intracellular messengers involved in the transduction of the external Ca2+ signal will be examined. Single cell spectrofluorimetry will be used to study the cytosolic Ca2+ responses to stimulation. Polyphosphoinositide and cyclic nucleotide systems will be examined, and G-protein involvement will also be evaluated. The Endocrinology Division at the Brigham and Women's Hospital consists of a unique combination of faculty, comprising both clinical and basic science investigators, and physical resources. Investigators here have a broad spectrum of expertise, including electrophysiology of endocrine tissues, single cell spectrofluorimetry, receptor physiology, molecular biology and cell biochemistry. Clinical investigators include experts in Ca2+ homeostasis and osteoporosis, hypertension, neuroendocrinology and circadian biology. This diverse group meets for several weekly research and clinical conferences. The members of this department have at their disposal several fully equipped modern laboratories and an administrative core. The resources of the Harvard Medical School and Brigham and Women Hospital are available, including the opportunity to participate in graduate school courses and consult with members of other departments.

这项建议的目的是阐明钙离子感应的机制。 一种对细胞外生理变化有反应的C细胞系 钙离子，RMTC 44-2。该细胞系是钙离子感应的良好模型， 尤其适用于内分泌组织的钙离子稳态 系统。我们将检查钙离子感应器的特异性。 使用除钙离子以外的多价阳离子。细胞内信使 参与外部钙信号的转导将被检测到。 将用单细胞荧光分光光度法研究细胞内钙离子 对刺激的反应。多磷肌醇和环核苷酸 系统将被检查，G-蛋白的参与也将被评估。 布里格姆妇女医院内分泌科由以下人员组成 一个独特的教员组合，包括临床和基础科学 调查人员和物力资源。这里的调查人员有一个广泛的 一系列专业知识，包括内分泌组织电生理学， 单细胞荧光测定法、受体生理学、分子生物学和 细胞生物化学。临床研究人员包括钙离子方面的专家 动态平衡与骨质疏松症、高血压、神经内分泌学 昼夜节律生物学这个不同的小组每周都会聚在一起进行几项研究 和临床会议。这个部门的成员在他们的办公室里 处置几个设备齐全的现代化实验室和一个行政 核心。哈佛医学院和布里格姆与女性的资源 医院都是可用的，包括有机会参加 研究生院的课程和咨询其他部门的成员。