RAPID IDENTIFICATION OF CLINICALLY SIGNIFICANT AIRBORNE FUNGAL SPORES

快速识别具有临床意义的空气传播真菌孢子

• 批准号: 3734206
• 负责人: JOHN E MAYFIELD
• 金额: --
• 依托单位: NORTH CAROLINA CENTRAL UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734206
• 关键词: Fungi; air sampling /monitoring; lasers; light microscopy; microbiology; microorganism classification; microorganism culture; monitoring device; particle; physical property; scanning electron microscopy; spores; technology /technique; technology /technique development

The long term objectives of the project involve the use of particle size analysis, scanning electron microscopy, light microscopy and fungal cultural techniques to develop both an extremely rapid and reliable procedure for both the identification and quantification of certain airborne fungal spores that are responsible for human health problems such as allergic disorders, infections and mycotoxins. The specific objectives are to: (1) develop appropriate techniques for the examination of spores with a particle size analyzer (PSA), (2) introduce quantitative parameters into the identification criteria of airborne fungal spores and (3) establish a detailed reference list of clinically important airborne fungal spores within the proximal atmosphere. To both verify the reliability of the PSA method and to establish certain baseline reference samples for identification parameters, a PSA, integrated with the IBM PC, will be utilized to first examine previously identified sporulating cultures. These established parameters will be used to identify spores collected from local air. The PSA will also be used to generate cumulative data about the size, density and distribution of spores. Since these methodologies will permit more frequent samplings, obtaining of air samples from a larger environment, a more rapid and accurate detection/identification procedure, and enhanced data processing and graphic representations, it is possible to both obtain a useful and comprehensive catalog of clinically significant airborne fungal spores and provide an established relationship between abundance of atmospheric spores and spore-related disease symptoms.

该项目的长期目标包括使用颗粒大小 分析、扫描电子显微镜、光学显微镜和真菌 栽培技术的发展既极其迅速又可靠 用于确定和量化某些物质的程序 导致人类健康问题的空气传播真菌孢子 例如过敏性疾病、感染和霉菌毒素。具体的 目标是：(1)开发适合考试的技术 用粒度分析仪(PSA)对孢子进行分析，(2)引入定量 气传真菌孢子和气传真菌孢子的鉴定 (3)建立详细的临床重要机载参考目录 近端大气中的真菌孢子。 为了验证PSA方法的可靠性，并建立一定的 用于识别参数的基准参考样本、PSA 与IBM PC集成，将被用来首先检查之前 鉴定了产孢子菌。这些已建立的参数将是 用来识别从当地空气中收集的孢子。PSA也将是 用于生成有关大小、密度和分布的累积数据 孢子的数量。因为这些方法将允许更频繁地 采样，从更大的环境中获取空气样本，更 快速、准确的检测/识别程序和增强的数据 处理和图形表示，则可以同时获得 有用和全面的临床上重要的空气传播目录 真菌孢子和丰度之间的关系已经确立 大气孢子和与孢子有关的疾病症状。