CLINICAL SIGNIFICANCE OF AIRBORNE POLLEN GRAINS AND FUNGAL SPORES

该项目的长期目标是提供一个 广泛的流行目录的流行， 空气中的真菌孢子和花粉粒，并发展可靠的 用于识别和量化的技术 某些真菌孢子和花粉的特殊变应原成分 晶粒 这项研究有三个组成部分。 （一） 建立真菌孢子的大气将 用安德森取样器收集孢子， 空气动力学地将孢子分成大小等级。 两 将利用文化特征和光学显微镜， 都能识别和量化空气中的真菌孢子 (2)隔离 和一些孢子特异性过敏原的特性 室内霉菌将完成使用SDS-凝胶电泳 和等速电泳技术。 另外，这其中的一个重要部分 该项目涉及识别和隔离的最 常见的室内霉菌。 (3)涉及电印迹的技术， 酶免疫测定法将用于鉴定全部补体 抗原，包括IgE结合抗原或过敏原 复杂的蛋白质混合物。 两种花粉的提取物 谷物和空气中的真菌孢子将被SOS凝胶分离 电泳，电印迹到硝酸纤维素膜上， 用过敏患者的血清、山羊抗人IgE和 进行免疫酶染色。 这种技术将是有用的 用于筛选大量过敏个体。 同源项目将提供所需的信息， 了解发病和严重程度之间的相关性 呼吸道过敏性疾病和某些花粉的流行 谷物和真菌孢子。 此外，他们将提供信息 为开发可靠的方法， 空气中过敏原的检测和定量。

The long-term objectives of this project are to provide an extensive catalog of the prevalence of the prevalence of both airborne fungal spores and pollen grains, and to develop reliable techniques for both the identification and quantification of specific allergenic components of certain fungal spores and pollen grains. There are three components of this study. (1) Establishment of fungal spores of the atmosphere will be accomplished by collecting spores with the Anderson Sampler that aerodynamically separates the spores into size classes. Both cultural characteristics and light microscopy will be utilized to both identify and quantify airborne fungal spores. (2) Isolation and characterization of some spores-specific allergens of some indoor molds will be accomplished by using SDS-gel electrophoresis and isotachophoresis techniques. Also, an important part of this project involves the identification and isolation of the most common indoor molds. (3) Techniques involving electroblotting and enzyme immunoassay will be utilized to identify the full complement of antigens, including IgE-binding antigens or allergens within complex mixtures of proteins. Certain extracts of both pollen grains and airborne fungal spores will be fractioned by SOS-gel electrophoresis, electroblotted onto nitrocellulose membranes and probed with sera from allergic patients, goat-antihuman-lgE and subjected to immunoenzyme staining. This technique would be useful for screening a large number of allergic individuals. The cognate projects will provide information that is needed to understand the correlation between the onset and severity of respiratory allergic disorders and the prevalence of certain pollen grains and fungal spores. Also, they will provide information necessary for the development of reliable methods for both the detection and quantification of airborne allergens.