RAPID IDENTIFICATION OF CLINICALLY SIGNIFICANT AIRBORNE FUNGAL SPORES

该项目的长期目标是确定 空气中的真菌孢子，并获得 了解孢子发育的机制，孢子 存活和孢子萌发影响临床上 重要的空气传播真菌孢子， 过敏性疾病、感染和霉菌毒素等问题。 的 具体目标是（1）改善和扩大粒径 基于分析仪的孢子快速鉴定方法，（2）增加 大气中临床和放射性同位素丰度信息库 与大气发生有关的发芽。 安徒生 将按月使用大气采样器，收集 孢子并对其进行粒度分析。 获得更好的 了解孢子发育、孢子存活和 相差、荧光扫描孢子萌发技术 电子和透射电子显微镜将用于观察 真菌野生型和突变株的某些关键阶段， 鬼伞 由于担孢子的同步发育， 易于在实验室中操作， 和有性孢子C. Cinereus将被用作建立 “创客”事件的发展、生存和萌芽。突变株 将用于突出关键阶段。 Cladosporium，一种常见的 收集和丰富的真菌，和曲霉菌频繁，但不是 丰富的真菌将与C.西纳勒斯 这些真菌 将暴露于模拟环境应力并进行分析 在细胞水平上的扰动。 因为知道 环境和内在因素影响空气真菌孢子 流行率将提供准确估计真菌感染的方法。 空气中的病原体。

The long-term objectives of the project is to determine the abundance of airborne fungal spores within the atmosphere and gain an understanding of the mechanism by which spore development, spore survival and spore germination influence prevalence of clinically significant airborne fungal spores that are responsible human health problems such as allergic disorders, infections and mycotoxins. The specific objectives are (1) improve and expand the particle size analyzer-based rapid spore identification method, (2) increase the information bank about the atmospheric abundance of clinically and germination relevant to atmospheric occurrence. The Andersen atmospheric sampler will be used on a monthly schedule, to collect spores and subject them to a particle size analysis. To obtain a better understanding of the extent that spore development, spore survival and spore germination techniques of phase contrast, florescent scanning electron and transmission electron microscopies will be used to observe certain critical stages in wild type and mutant strain of the fungus, Coprinus cinereus. Because of its synchronous basidiospore development, ease of manipulation in the laboratory and production of both asexual and sexual spores, C. Cinereus will be used as a model for establishing "maker" events in development, survival and germination. Mutant strains will be used to highlight critical stages. Cladosporium, a frequently collected and abundant fungus, and Aspergillus a frequent but not abundant fungus will be studied along with C. Cinereus. These fungi will be exposed to simulated environmental stresses and analyzed perturbations at the cellular level. Knowing the extent to which environmental and intrinsic factors influence airborne fungal spore prevalence will provide the means for accurate estimates of fungal disease agents in the atmosphere.