NEW SENSORS BASED ON MOLECULAR RECOGNITION IN MONOLAYERS

该提案的目标是制定一个通用的方法， 生物分子的化学选择性传感器。 为此，分子 自组装单层中的识别位点将通过 在模板分子周围组装单层成分， 分子间相互作用（离子键或氢键）。后续 通过冲洗或切割除去模板分子 在识别位点后面用于模板的重吸收，或者非常 相似的分子。 吸附分子的红外光谱和 比较类似分子的吸附等温线将有助于 阐明识别位点特异性背后的原因。 分子 含有识别位点的传感器将利用 厚度剪切模式谐振器 避免非特异性干扰 表面污染，谐振器的频率和衰减 将在分子吸附过程中进行监测，因为衰减较小 可能被非特异性吸附改变。 通过结合识别 具有选择性检测的部位特异性，鉴别传感器应 实现。 这种传感器将证明在诊断（监测）中是有用的 生物中间体）以及临床应用（监测 药物递送）。

The goal of this proposal is to develop a general method for the creation of chemically selective sensors for biomolecules. To this end, molecular recognition sites in self-assembled monolayers will be created by assembling monolayer constituents around template molecules via intermolecular interactions (ionic or hydrogen bonding). Subsequent removal of the template molecules by rinsing or cleavage will leave behind a recognition site for reabsorption of the template or very similar molecules. Infrared spectra of the adsorbed molecules and comparison of the adsorption isotherms of similar molecules will help elucidate the reasons behind recognition site specificities. Molecular sensors containing the recognition sites will then be developed using thickness shear mode resonators. To avoid interferences from nonspecific surface contamination, both frequency and attenuation of the resonator will be monitored during molecular adsorption because attenuation is less likely to be changed by nonspecific adsorption. By combining recognition site specificity with selective detection, discriminating sensors should be realized. Such sensors would prove useful in diagnostic (monitoring biological intermediates) as well as clinical applications (monitoring drug delivery).