GOALI: Development of a New Generation of Sensors based on Metal/Bacteria Interfaces

GOALI：开发基于金属/细菌界面的新一代传感器

• 批准号: 0702200
• 负责人: Rhonda Franklin
• 金额: $ 30万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0702200&HistoricalAwards=false
• 关键词: GOALI; Development; New; Generation; Sensors

The objective of this research is to develop a framework for a new generation of bio-sensors harnessing bacteria able to transfer electrons to electrodes, by fabricating novel electrode systems able to study, define, and manipulate the pathway of electron transfer between these unique bacteria and electrode surfaces. Specific aims include determination of (a) biofilm resistance at bacteria-electrode interfaces, (b) electron transfer rates to electrodes with nano- versus microscale surface roughness, and (c) long-range conductance between bacteria. This will allow engineering of prototype sensors that exploit the specificity and diversity of bacterial metabolism, while utilizing lower-cost fabrication approaches through industrial partnerships.The intellectual Merit of this research is in the development of models predicting electronic properties and performance of bacterial films. Test electrodes optimized for bacterial interactions will improve data reliability when studying bacterial biofilms, and reveal fundamental information about the nature of microbial electron transfer. The Broader Impact of this research involves its potential to improve and reduce the cost of multiple applications related to microbial activity at electrodes, including; environmental monitoring with bio-based devices, accelerated bioremediation, and renewable energy production. This project will involve a diverse (age, gender, and ethnicity) workforce who will play a key role in technology transfer through university research as well as internships with this GOALI's industrial partner. Educational modules on "green" electronics will be generated, and public STEM education forums (e.g. Science Museum of Minnesota) will be used to target broader and under-represented K-12 community groups.

这项研究的目的是通过制造能够研究，定义和操纵这些独特细菌和电极表面之间电子传递的途径来利用能够将电子传递到电极的新一代生物传感器的框架。具体目的包括在细菌 - 电极界面上确定（a）生物膜耐药性，（b）电子转移速率到具有微观表面粗糙度的电极，以及（c）细菌之间的远距离电导率。这将允许设计原型传感器的工程，从而利用细菌代谢的特异性和多样性，同时通过工业伙伴关系利用低成本制造方法。这项研究的知识优点在于开发预测电子特性和细菌膜性能的模型。在研究细菌生物膜时，针对细菌相互作用进行优化的测试电极将提高数据可靠性，并揭示有关微生物电子转移性质的基本信息。这项研究的更广泛影响涉及改善和降低与电极上微生物活动相关的多种应用的成本的潜力，包括：使用基于生物的设备，加速生物修复和可再生能源生产的环境监测。该项目将涉及多样化（年龄，性别和种族）劳动力，他们将通过大学研究以及与该Goali的工业合作伙伴实习在技术转移中发挥关键作用。 将生成有关“绿色”电子产品的教育模块，公共STEM教育论坛（例如明尼苏达州科学博物馆）将用于针对更广泛和代表性不足的K-12社区团体。