STRUCTURES OF DNA AND RNA QUADRUPLEXES

DNA 和 RNA 四链体的结构

• 批准号: 2172683
• 负责人: NICHOLAS V. HUD
• 金额: $ 2.99万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-01-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2172683
• 关键词: STRUCTURES; DNA; RNA; QUADRUPLEXES

Purine-rich nucleotide sequences have been implicated in a number of important biological processes. These include roles in eucaryotic chromosome replication, gene regulation, meiosis and the retrovirus replication cycle. Over the past several years it has become clear that at least some of these functions are intimately connected with the ability for purine-rich oligonucleotides to form quadruplex structures that are stabilized by G-quartets. In the work proposed here, NMR spectroscopy will be used to determine the high resolution solution structures of several DNA and RNA oligonucleotides which are known, or suspected to, form G-quartets under physiological conditions. The oligonucleotides selected for study are intended to answer specific questions related the higher order structures of DNA and RNA sequences found in telomeres and retrovirus genomes, respectively. The long term goals of the DNA studies are to understand the normal functionality of telomeres in chromosome replication and cell division. The RNA studies are directed towards an increased understanding of the retrovirus replication cycle and identification of promising targets for anti-viral agents.

富含嘌呤的核苷酸序列已经涉及许多 重要的生物学过程。这些包括在真核生物中的作用， 染色体复制、基因调控、减数分裂和逆转录病毒 复制周期在过去的几年里，很明显， 这些功能中至少有一部分与 富含嘌呤的寡核苷酸形成四链体结构的能力 由G四重奏稳定 在这里提出的工作中，NMR 将使用光谱法测定高分辨率溶液 已知的几种DNA和RNA寡核苷酸的结构，或 怀疑在生理条件下形成G-四联体。的 选择用于研究的寡核苷酸旨在回答特定的 与DNA和RNA序列的高级结构有关的问题 分别存在于端粒和逆转录病毒基因组中。长期 DNA研究的目的是了解正常的功能， 端粒在染色体复制和细胞分裂中的作用。RNA研究 旨在加深对逆转录病毒的了解 复制周期和有希望的抗病毒靶点的鉴定 剂.