NUDH NUCLEAR MIGRATION GENE

细胞核在细胞内的迁移发生在大多数，如果不是全部的话， 真核生物。核迁移是聚变所必需的 在受精和胚胎发育过程中的原核。那里 也有证据表明它可能参与大脑发育和 癌细胞的转移。尽管基本的重要性在于 核迁移，对其监管几乎一无所知或 机械师。我们建议通过描述核迁移的特征来研究核迁移 尼杜拉曲霉的nudH基因。对温度敏感的基因突变 这种基因抑制了核迁移。我们将通过以下方式克隆nudH基因 其温度敏感性的互补，2)基因测序，3) 分析nudF基因产物的功能，以及4)鉴定和 鉴定其产物与NUDF蛋白相互作用的基因。

Migration of nuclei within cells occurs in most, if not all, eukaryoticorganisms. Nuclear migration is required for the fusion of pronuclei during fertilization and for embryological development. There is also evidence that it may be involved in brain development and metastasis of cancer cells. Despite the fundamental importance of nuclear migration, almost nothing is known about its regulation or mechanics. We propose to study nuclear migration by characterizing the nudH gene of Aspergillus nidulans. Temperature sensitive mutations in this gene inhibit nuclear migration. We will 1) clone the nudH gene by complementation of its temperature sensitivity, 2) sequence the gene, 3) analyze the function of the nudF gene product, and 4) identify and characterize genes whose products interact with the NUDF protein.