NUDH NUCLEAR MIGRATION GENE

NUDH 核迁移基因

• 批准号: 2518816
• 负责人: BO LIU
• 金额: $ 2.53万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2518816
• 关键词: cell cycle; cell nucleus; cytogenetics; fungal genetics

Migration of nuclei within cells occurs in most, if not all, eukaryoticorganisms. Nuclear migration is required for the fusion of pronuclei during fertilization and for embryological development. There is also evidence that it may be involved in brain development and metastasis of cancer cells. Despite the fundamental importance of nuclear migration, almost nothing is known about its regulation or mechanics. We propose to study nuclear migration by characterizing the nudH gene of Aspergillus nidulans. Temperature sensitive mutations in this gene inhibit nuclear migration. We will 1) clone the nudH gene by complementation of its temperature sensitivity, 2) sequence the gene, 3) analyze the function of the nudF gene product, and 4) identify and characterize genes whose products interact with the NUDF protein.

细胞核在细胞内的迁移发生在大多数，如果不是全部， 真核生物核聚变需要核迁移， 原核在受精和胚胎发育。 那里 也证明了它可能与大脑发育有关， 癌细胞的转移。 尽管基本的重要性， 核迁移，几乎没有什么是知道它的监管或 力学 我们建议研究核迁移的特点， nudH基因。 温度敏感突变 该基因抑制核迁移。 我们将1）克隆nudH基因， 互补其温度敏感性，2）测序基因，3） 分析nudF基因产物的功能; 4）鉴定和 表征其产物与NUDF蛋白相互作用的基因。