Evaluating spectroscopic imaging of living skin equivalents as a new approach for topical formulations research

评估活体皮肤等效物的光谱成像作为局部制剂研究的新方法

• 批准号: 131719
• 金额: $ 21.18万
• 依托单位: NPL MANAGEMENT LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=131719
• 关键词: Evaluating; spectroscopic; imaging; living; skin

In vitro product testing is a vital part of product development for topical applications and devices and this project seeks to establish a powerful non-animal approach as a new method for providing a greater understanding of percutaneous absorption of topical formulations in pre-clinical studies. This project aims to develop label free imaging of drugs by mass spectrometry imaging (MSI) and stimulated Raman spectroscopy (SRS) in human living skin equivalents (HSLEs). We propose to undertake the first comparison of each of these techniques for imaging of drugs and to gather data to allow a robust evaluation of the penetration profiles of pharmaceutical compounds in human and pig ex vivo skin, and in an HLSE. In addition, we will introduce significant novelty and new metrology to support the application of the HLSEs and spectroscopic imaging to provide reliable and transferable protocols. The ultimate aim of this project is to support the 3Rs via the delivery of a non-animal model and novel chemical imaging protocols which are validated for topical formulation testing to allow reliable predictions of safety and efficacy in clinical studies.

体外产品测试是局部应用和器械产品开发的重要组成部分，该项目旨在建立一种强大的非动物方法，作为一种新方法，用于在临床前研究中更好地了解局部制剂的经皮吸收。本研究旨在开发无标记的药物质谱成像技术（MSI）和受激拉曼光谱（SRS）。我们建议对这些药物成像技术进行首次比较，并收集数据，以便对药物化合物在人和猪离体皮肤和HLSE中的渗透特性进行稳健评价。此外，我们将引入重要的新奇和新的计量学来支持HLSE和光谱成像的应用，以提供可靠和可转移的协议。该项目的最终目的是通过提供非动物模型和新型化学成像方案来支持3R，这些方案经验证可用于局部制剂测试，从而可以可靠地预测临床研究中的安全性和有效性。