Discovery and development of large/diverse user-friendly panels of novel biocatalysts

发现和开发大型/多样化的用户友好的新型生物催化剂面板

• 批准号: 131839
• 金额: $ 8.94万
• 依托单位: PROZOMIX LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=131839
• 关键词: Discovery; development; large; diverse; user

This project aims to use cutting-edge high-throughput proprietary bioinformatics software, called "Filter-BLAST", in conjunction with the ever rapidly expanding DNA databases, to efficiently and accurately identify at the in silico level 500 key target enzymes, representing a diversity of 10 key biochemistries currently missing from the synthetic chemists enzyme toolbox. Advanced/proprietary high-throughput cloning technology (previously developed by Prozomix using TSB funds), called "GRASP", will then be used to rapidly clone the target genes selected and develop the encoded biocatalysts to cost-effectively populate 10 large/novel screening panels of key enzymes, and provide these materials to the biocatalysis community for screening towards specific individual biocatalysts of interest. After discovering enzymes fitting their exacting requirements, commercial quantities in animal-free, BSE-TSE certified form from Prozomix will be used to establish the new IB applications in the UK.

该项目旨在使用尖端的高通量专有生物信息学软件，称为“Filter-BLAST”，结合不断快速扩展的DNA数据库，在计算机水平上有效和准确地识别500种关键靶酶，代表了目前合成化学家酶工具箱中缺少的10种关键生物化学的多样性。先进的/专有的高通量克隆技术（以前由Prozomix使用TSB基金开发），称为“GRASP”，然后将用于快速克隆选定的靶基因，并开发编码的生物催化剂，以具有成本效益地填充10个大型/新型关键酶筛选板，并将这些材料提供给生物催化界，用于筛选特定的感兴趣的生物催化剂。在发现符合其严格要求的酶后，Prozomix的无动物BSE-TSE认证形式的商业数量将用于在英国建立新的IB申请。