A Systems Biology Approach to the Optimisation of (Fed-) Batch and Continuous Fermentation Processes for Recombinant Protein Production

该可行性项目的目的是通过使用代谢组学和蛋白质组学的复杂分析技术，在生物催化剂的大规模和高滴度生产所需的一系列（补料）分批和连续发酵条件下，对生物体的压力进行深入的系统生物学水平的理解。该项目旨在了解用于指导蛋白质生产的遗传结构和工艺条件的变化如何影响生物体，以及如何通过进一步重新设计遗传组分，饲料培养基和工艺条件来减轻，最小化和控制这些影响。通过缩短周期时间和提高原材料效率，开发稳健的发酵工艺具有经济优势，并且通过向工业生物技术使用行业提供可靠且更经济的酶商业供应，将对本可行性研究产生明显影响。

The purpose of this feasibility project is to create a deep systems biology level understanding of the stresses placed upon an organism, under a range of (fed-)batch and continuous fermentation conditions required for the large scale and high titre manufacture of biocatalysts, through the use of the complex analytical techniques of metabolomics and proteomics. The project seeks to understand how variation in the genetic constructs and process conditions used to direct protein production can affect the organism and how these effects might be mitigated against, minimised and controlled by further re-design of the genetic components, feed medium and process conditions. The development of robust fermentation processes has economic advantages, through both cycle time reduction and raw material efficiencies and will have clear impact beyond this feasibility study through the reliable and more economic commercial supply of enzymes to Industrial Biotechnology using industries.