Rapid and cost effective ‘on-site’ detection of Cacao swollen-shoot virus (CSSV).

快速、经济高效的“现场”检测可可肿芽病毒 (CSSV)。

• 批准号: 132346
• 金额: $ 27.35万
• 依托单位: MARS WRIGLEY CONFECTIONERY UK LIMITED
• 依托单位国家: 英国
• 项目类别: BEIS-Funded Programmes
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=132346
• 关键词: Rapid; cost; effective; site; detection

Cocoa beans are the key raw material in chocolate manufacturing. In West Africa, where 70% of thecocoa imported and consumed in the UK is produced, the chocolate industry has identified CacaoSwollen Shoot Disease (CSSV) as the major constraint to productivity. A cacao farm with CSSV will sufferdeclining yields and hence declining farmer income from what is often the only cash crop being grown.To limit the spread of this disease it is essential to develop diagnostic test that can detectpresymptomatic infection facilitating its prompt control. In this proposal we will develop a suitablemethodology and associated instrumentation to detect CSSV in crude plant extracts. Our approach willemploy paper based detection assays suitable for rapid application that can be used in formats thatenable unskilled personnel to operate them, thereby enabling their widespread deployment in the field.This will have a significant impact on the economic prospects of cacao farmers in West Africa.

可可豆是巧克力制造的关键原材料。英国进口和消费的可可中 70% 是在西非生产的，巧克力行业已将可可肿芽病 (CSSV) 视为生产力的主要限制因素。患有 CSSV 的可可农场将遭受产量下降，从而导致农民收入下降，因为可可种植的唯一经济作物往往是唯一的经济作物。为了限制这种疾病的传播，必须开发能够检测症状前感染的诊断测试，从而促进其及时控制。在本提案中，我们将开发一种合适的方法和相关仪器来检测粗植物提取物中的 CSSV。我们的方法将采用适合快速应用的纸质检测方法，可以以非熟练人员操作的形式使用，从而使其能够在田间广泛部署。这将对西非可可种植者的经济前景产生重大影响。