STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
基本信息
- 批准号:2179935
- 负责人:
- 金额:$ 12.57万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-06-01 至 1997-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The objectives of this grant are to characterize the unique structure of
the LPS from R. leguminosarum biovar phaseoli (recently reclassified
Rhizobium etli) strain CE3 and to elucidate the role of this molecule in
the nitrogen-fixing symbiotic infection of its legume host it is known
that Rhizobium LPS are required for a successful infection of the legume
host. Gross changes in the LPS, e.g. loss of the O-chain polysaccharide,
result in aborted infections. Monoclonal antibodies have shown that
during infection there are subtle structural changes that occur in the LPS
during infection. By using defined mutants in the LPS biosynthetic pathway
(provided by Dr. Dale Noel of Marquette University), the complete
structure of the LPS will be determined, as well as those epitope changes
that occur during symbiotic infection. Analysis of LPSs from mutants at
various stages of the infection process will (a) facilitate the
identification of genes in the LPS biosynthetic pathway, and (b) enable us
to determine how the O-chain, core oligosaccharides and lipid A linked in
the complete LPS molecule. The LPS from these mutants, as well as from the
parent strain (CE3) will be used to isolate those LPS fragments that carry
the epitopes important in symbiotic infection. These epitopes will be
identified by ELISA inhibition assays. The monoclonal antibodies (i.e.
JIM26, JIM27, JIM28 and JIM29) have been provided by Dr. Nick Brewin of
the John Innes Institute in the UK. Additionally, a graduate student will
visit Dr. Noel's laboratory to isolate mutants that are unable to undergo
those epitope changes that occur during symbiotic infection. These mutants
will be analyzed to determine their structural (by us) and symbiotic (by
Dr. Noel) defects. Structural determination of the various LPSs and LPS
fragments will be carried out by glycosyl composition and methylation
analyses, NMR, and high resolution mass spectrometry. We currently know
the structures of the O-chain repeating unit, two core oligosaccharides,
and the lipid A from the R. leg. bv. phaseoli CE3 LPS. The lipid A of
Rhizobium is unique in that it does not have phosphate, and its sugar
backbone consists of galacturonic acid, glucosamine and 2-aminogluconic
acid. We have shown that Rhizobium extracts have all the enzymes necessary
for the synthesis of a lipid A precursor common to enteric lipid As,
Kdo2IVa. Thus, in Rhizobium this common precursor is probably processed to
form the unique rhizobial lipid A. One such enzyme, a specific Kdo2IVa 4'-
phosphatase has already been identified. The last objective of this
proposal is to characterize the other enzymes that process Kdo2lVa into
the unique rhizobial lipid A. Dr. Chris Raetz (Duke University) has agreed
to collaborate with us in this aspect of the project.
这项奖助金的目的是描述
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RUSSELL W CARLSON其他文献
RUSSELL W CARLSON的其他文献
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{{ truncateString('RUSSELL W CARLSON', 18)}}的其他基金
Rhizobial Lipopolysaccharides Essential for Infection
感染必需的根瘤菌脂多糖
- 批准号:
8008946 - 财政年份:2010
- 资助金额:
$ 12.57万 - 项目类别:
Biosynthesis & Function of a Bacillus anthracis-specific cell wall polysaccharide
生物合成
- 批准号:
7739417 - 财政年份:2009
- 资助金额:
$ 12.57万 - 项目类别:
Biosynthesis & Function of a Bacillus anthracis-specific cell wall polysaccharide
生物合成
- 批准号:
7860446 - 财政年份:2009
- 资助金额:
$ 12.57万 - 项目类别:
Bacillus anthracis cell surface carbohydrates
炭疽芽孢杆菌细胞表面碳水化合物
- 批准号:
6803538 - 财政年份:2003
- 资助金额:
$ 12.57万 - 项目类别:
Bacillus anthracis cell surface carbohydrates
炭疽芽孢杆菌细胞表面碳水化合物
- 批准号:
6673663 - 财政年份:2003
- 资助金额:
$ 12.57万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
2179936 - 财政年份:1988
- 资助金额:
$ 12.57万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
2179937 - 财政年份:1988
- 资助金额:
$ 12.57万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
3296709 - 财政年份:1988
- 资助金额:
$ 12.57万 - 项目类别:
Rhizobial Lipopolysaccharides Essential for Infection
感染必需的根瘤菌脂多糖
- 批准号:
6624026 - 财政年份:1988
- 资助金额:
$ 12.57万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
3296705 - 财政年份:1988
- 资助金额:
$ 12.57万 - 项目类别:
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