RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION

描述：本修订申请是为了支持对 脂多糖组分的结构和生物合成 参与节肢动物共生的分子。这些细胞壁多糖 被认为是毒力的重要决定因素，并被要求 对宿主的入侵负责。总体目标是确定 这些复合糖脂的结构和生物合成，并确定 生物活性所需的特定结构特征。这个 具体目标是：1)表征最小LipidA核心结构 O-链多糖的连接要求，以及 描述对这种最低限度的脂类的修饰。核心结构 这导致增加的O链多糖的连接，2)到 描述在过程中发生的O-链的结构修饰 共生侵染，3)分离豆类根霉突变体 无法添加在LPSs中常见的独特结构特征 该种，以及4)豆类根霉特异性酶的特征。 这一过程将Kdo2-Liped-IVA前体转化为这个独特的结构 有机体。

DESCRIPTION: This revised application is to support the investigation of the structure and biosynthesis of the polysaccharide components of the LPS molecules involved in Rhyzobial symbiosis. These cell wall polysaccharides are thought to be important determinants of virulence and to be required for the invasion of the host. The overall goal is to determine the structure and biosynthesis of these complex glycolipids and determine the specific structural features required for their biological activity. The specific aims are: 1) characterize the minimum lipidA core structure requirements for the ligation of the O-chain polysaccharide, and characterize the modification to this minimal lipid. A core structure which lead to the ligation of increased O-chain polysaccharide, 2) to characterize the structural modification to the O-chain that occur during the symbiotic infections, 3) to isolate mutants of R. leguminosarum that are unable to add unique structural features normal found in the LPS of this species, and 4) to characterize the R. leguminosarum specific enzymes that process the Kdo2-lipid-IVA precursor to the unique structure of this organism.