Rhizobial Lipopolysaccharides Essential for Infection
感染必需的根瘤菌脂多糖
基本信息
- 批准号:6730568
- 负责人:
- 金额:$ 26.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-06-01 至 2006-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Rhizobia are Gram-negative soil bacteria
that form nitrogen-fixing symbioses with legumes. A specific rhizobial species
recognizes and infects a specific host plant. Rhizobial cell surface
lipopolysaccharides (LPSs) are important for endocytotic invasion of host root
nodule cells and differentiation into nitrogen-fixing bacteroids within a
host-derived intracellular compartment known as the symbiosome. The symbiosome
membrane is derived from the Golgi and endoplasmic reticulum of the host cell
and is both acidic and low in O2. Rhizobial symbionts are analogous to animal
bacterial pathogens that survive in phagosome-derived intracellular
compartments and cause chronic infections; e.g. Brucella. The LPSs from members
of the Rhizobiaceae have a very long chain lipid-A fatty acid, 27-OHC28:0, (as
does the pathogen, Brucella abortus). Also, LPSs from R. etli (Re) (a bean
symbiont) and from R. leguminosarum bv. vicae (Rlv) (a pea symbiont) have
unique lipid-A, and core oligosaccharide; e.g. they are devoid of phosphate and
contain galacturonic acid. During symbiosis, modifications are made to the
O-chain polysaccharide (e.g. methylation) and lipid-A. In the case of Rlv, both
LPS and the entire bacterium become hydrophobic. This increase in
hydrophobicity is due primarily to a doubling of the lipid-A 27-OHC28:0 moiety.
It is proposed that the LPS O-chain modifications are required for adherence of
the bacterium to the host membrane, and that the 27-OHC28:0 moiety is required
to maintain membrane stability during endocytosis and symbiosome formation. The
aims of this proposal are to structurally characterize the O-chain
modifications, and to determine the functions of the Re and Rlv unique
structural features with regard to both symbiosis and LPS biosynthesis. This
will be accomplished through the creation and analysis of Re and Rlv mutants
that are specifically altered in these unique structural features (e.g.
defective in 27-OHC28:0 incorporation), and by the isolation and
characterization of LPS fragments that bind to specific monoclonal antibodies
whose LPS epitopes change during symbiosis.
描述(由申请人提供):根瘤菌是革兰氏阴性土壤细菌
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RUSSELL W CARLSON其他文献
RUSSELL W CARLSON的其他文献
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{{ truncateString('RUSSELL W CARLSON', 18)}}的其他基金
Rhizobial Lipopolysaccharides Essential for Infection
感染必需的根瘤菌脂多糖
- 批准号:
8008946 - 财政年份:2010
- 资助金额:
$ 26.94万 - 项目类别:
Biosynthesis & Function of a Bacillus anthracis-specific cell wall polysaccharide
生物合成
- 批准号:
7739417 - 财政年份:2009
- 资助金额:
$ 26.94万 - 项目类别:
Biosynthesis & Function of a Bacillus anthracis-specific cell wall polysaccharide
生物合成
- 批准号:
7860446 - 财政年份:2009
- 资助金额:
$ 26.94万 - 项目类别:
Bacillus anthracis cell surface carbohydrates
炭疽芽孢杆菌细胞表面碳水化合物
- 批准号:
6803538 - 财政年份:2003
- 资助金额:
$ 26.94万 - 项目类别:
Bacillus anthracis cell surface carbohydrates
炭疽芽孢杆菌细胞表面碳水化合物
- 批准号:
6673663 - 财政年份:2003
- 资助金额:
$ 26.94万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
2179936 - 财政年份:1988
- 资助金额:
$ 26.94万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
2179937 - 财政年份:1988
- 资助金额:
$ 26.94万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
3296709 - 财政年份:1988
- 资助金额:
$ 26.94万 - 项目类别:
Rhizobial Lipopolysaccharides Essential for Infection
感染必需的根瘤菌脂多糖
- 批准号:
6624026 - 财政年份:1988
- 资助金额:
$ 26.94万 - 项目类别:
STRUCTURAL DETERMINATION OF THE LPS FROM RHIZOBIUM
根瘤菌 LPS 的结构测定
- 批准号:
2179935 - 财政年份:1988
- 资助金额:
$ 26.94万 - 项目类别:
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