SATURATION MAPPING OF HUMAN CHROMOSOME 3

该计划项目的目标是制作详细的物理和 人类2号和3号染色体多态标记的遗传图谱 目前的2号和3号染色体图谱只有有限数量的标记。 而且在物理和基因图谱上都有几个很大的差距。 计划项目生成的物理地图将基于 辐射减量杂交种及其遗传图谱的CEPH分析 家族DNA。产生的遗传标记都将基于聚合酶链式反应和 由高度多态的标记组成，例如用简单的二，三-， 和四核苷酸重复序列，以及在L1序列的聚A区中 作为这些染色体特有的转录序列。此外， 程序将设计一种方法，将每条染色体与Line进行全局映射 元素，并将这一图谱与遗传和辐射杂交图谱相关联。 为了实现这些目标，该方案由4个项目和一个核心项目组成 用于联系研究以及行政核心。这些高度 相互依赖的项目将导致600个非常有用的项目的安置 授权期内2号和3号染色体上的标记。记号笔是 被称为高度有用，因为它们将是高度多态的，基于PCR 并可供所有调查人员使用，并在物理和 基因图谱。生成的物理地图将在2厘米处完成 水平和在许多地区将实现1厘米的距离。体能 每条染色体的图谱将在每兆数据库中有大约一个标记。这 将使用标记和细胞试剂的框架小组来指导 分离2号和3号染色体的有序克隆并将特别 对这些染色体的闭合很有用。多态标记将是 立即适用于识别疾病部位附近的标记。

The goal of this program project is to produce detailed physical and genetic maps of polymorphic markers for human chromosomes 2 and 3. The current maps of chromosomes 2 and 3 only have a limited number of markers and there are several large gaps on both the physical and genetic maps. The physical map generated by the program project will be based on radiation reduction hybrids and the genetic map a result of analyzing CEPH family DNA. The genetic markers generated will all be PCR based and consist of highly polymorphic markers such as found with simple di-, tri-, and tetranucleotide repeats and in the poly A tract of L1 sequences as well as transcribed sequences specific for these chromosomes. In addition the program will devise a method for globally mapping each chromosome with Line elements and correlating this map to the genetic and radiation hybrid map. To accomplish these goals, the program consists of 4 projects and a core for linkage studies as well as an administrative core. These highly interdependent projects will result in the placement of 600 highly useful markers on chromosomes 2 and 3 over the grant period. The markers are termed highly useful since they will be highly polymorphic, be based on PCR and available to all investigators, and be mapped on both physical and genetic maps. The physical map generated will be complete at the 2 cM level and in many regions 1 cM distances will be achieved. The physical map of each chromosome will have approximately 1 marker per megabase. This framework panel of markers and cell reagents will be used to direct the isolation of ordered clones for chromosomes 2 and 3 and will be especially useful for closure on these chromosomes. The polymorphic markers will be immediately applicable for identifying markers adjacent to disease loci.