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GENOMICS SHARED RESOURCE

GENOMICS SHARED RESOURCE
基因组学共享资源
批准号:
7944742
负责人:
SUSAN L NAYLOR
金额:
$5.23万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-08-03 至 2012-07-31
关键词:
AcidsAliquotAnticoagulantsArabidopsisArtsAspirate substanceBiological AssayBiological MarkersBloodBlood specimenBone MarrowCaenorhabditis elegansCancer CenterCanis familiarisCattleCell LineCellsChickensCitratesConsultCulture MediaCultured CellsCustomCytogenetics Shared ResourceDNADNA copy numberDataData QualityDatabasesDiagnosisDiagnostic Neoplasm StagingDiscriminationDrosophila genusExperimental DesignsFluorochromeFreezingFundingGene DeletionGene DosageGene ExpressionGene Expression Microarray AnalysisGenesGeneticGenetic PolymorphismGenomeGenomicsGenomics Shared ResourceGenotypeGlucoseHumanHuman GenomeHuman Herpesvirus 4IndustryLabelLengthLymphocyteMacaca mulattaMaizeManualsMessenger RNAMethodologyMethodsMicroarray AnalysisMicroarray Shared ResourceMolecularMolecular GeneticsMolecular ProfilingMononuclearMononuclear LeukocytesMusNucleic AcidsOligonucleotidesPeripheral Blood Mononuclear CellPrimer ExtensionProcessPromegaProtocols documentationRNARanaRattusReaderRelative (related person)ReproducibilityResearch PersonnelResource SharingResourcesRiceRunningSamplingServicesSingle Nucleotide PolymorphismSolid NeoplasmSourceSpecimenSpeedTechnical ExpertiseTechnologyTestingTherapeutic StudiesTimeTubeTumor Cell LineTumor TissueVenousVial deviceViralWhole BloodYeastsZebrafishanticancer researchbasecancer cellcancer therapycomparative genomic hybridizationcongeniccostcyanine dye 5densityexperiencegenetic technologyimmortalized cellinstrumentinterestlymphoblastoid cell linememberneoplastic cellperipheral bloodresearch studysextissue culturetissue/cell culturetrizoltumor

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中文摘要
翻译
基因组学共享资源为癌症中心的成员提供最先进的服务, 提供了两个互补平台,即主要用于高通量的Illumina平台, 单核苷酸多态性的基因分型和Agilent Technologies平台,使用 主要用于阵列比较基因组杂交和表达微阵列分析。除了 这些基因组服务,共享资源为癌症中心成员提供自动化的帮助, 核酸分离、实时定量PCR和样品库。样本库的范围从 分离DNA,处理血液标本,建立淋巴母细胞系和长期储存 样品对共享资源进行了重组,由一名总协调员和两名共同主任负责, 每个平台一个。这三个人都在基因组学方面拥有丰富的经验,并为癌症中心成员提供 提供咨询服务以协助实验设计。此共享资源结合了以前的两个 资助的癌症中心共享资源,细胞遗传学和遗传学共享资源和微阵列 共享资源。
英文摘要
The Genomics Shared Resource provides state-of-the-art services to the members of the Cancer Center by providing access to two complementary platforms, the lllumina platform, used for primarily for highthroughput genotyping of single nucleotide polymorphisms, and the Agilent Technologies platform, used primarily for array comparative genomic hybridization and expression microarray analysis. In addition to these genomic services, the shared resource offers Cancer Center members assistance in automated nucleic acid isolation, real-time quantitative PCR, and sample banking. The banking of samples range from isolating DNA, processing blood specimens, establishing lymphoblastoid cell lines and long term storage of samples. The shared resource has been reorganized with a single overall coordinator and two co-directors, one per platform. All three have extensive experience in genomics and provide Cancer Center members with consulting services to assist in experimental design. This shared resource combines two previous funded cancer center shared resources, the Cytogenetic and Genetics Shared Resource and the Microarray Shared Resource.
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