PEPTIDE SECONDARY METABOLITE BIOSYNTHESIS IN BACILLUS

芽孢杆菌中肽次级代谢产物的生物合成

• 批准号: 2183502
• 负责人: PETER ZUBER
• 金额: $ 12.12万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2183502
• 关键词: Bacillus; Escherichia coli; antibiotics; bacterial genetics; developmental genetics; gene expression; genetic manipulation; genetic mapping; genetic promoter element; genetic transcription; growth media; mutant; nucleic acid sequence; operon; peptides; protein biosynthesis; surfactant; transposon /insertion element

Unicellular organisms as well as the cells of higher organisms utilize low molecular weight polypeptides as extracellular signals that regulate cell differentiation and specialization. Spore- forming bacteria of the genus Bacillus are known to produce a number of peptides which possess antimicrobial activity. More recently, evidence has emerged which implicates these peptides as regulators of sporulation and competence development. Surfactin is a cyclic lipopeptide and powerful biosurfactant produced by B. subtilis. Genes required for surfactin production have been identified and isolated. These include the srfA operon, which encodes at least one of the enzymes involved in the synthesis of surfactin. Genetic evidence has shown that srfA is also required for competence development and efficient sporulation, which suggests that peptides synthesized by the srfA enzymes may function in developmental processes. Transcription of srfA is activated in stationary phase cells and is repressed by readily metabolized carbon and nitrogen sources. Production of surfactin also requires the comA gene, a putative transcriptional regulator of competence gene and srfA transcription. The sfp gene is also required for surfactin production, but its primary function of is unknown. The goals of the experiments described herein is to identify and characterize the products of the srfA and sfp genes, and to elucidate the mechanisms of srfA transcriptional regulation. Genes that require srfA for their expression will also be identified in order to begin to examine the role of srfA in Bacillus development.

单细胞生物以及较高生物体的细胞利用低 分子量多肽作为调节细胞的细胞外信号 分化和专业化。 孢子形成属的细菌 已知芽孢杆菌会产生许多拥有的肽 抗菌活性。 最近，出现了证据 将这些肽视为孢子和能力的调节剂 发展。 表面素是一种环状脂肪肽和强大的生物表面活性剂 由枯草芽孢杆菌生产。 表面生产所需的基因已经 确定和孤立。其中包括SRFA操纵子，该操纵子编码 至少有一种参与表面素的酶。 遗传 证据表明，能力发展也需要SRFA 和有效的孢子形成，这表明由 SRFA酶可能在发育过程中起作用。 转录的 SRFA在固定相细胞中被激活，并容易被抑制 代谢碳和氮源。 也产生表面素 需要昏迷基因，这是一种推定的能力转录调节器 基因和SRFA转录。 表面素也需要SFP基因 生产，但其主要功能是未知的。 目标的目标 本文所述的实验是识别和表征产品 SRFA和SFP基因，并阐明SRFA的机制 转录调节。 需要SRFA表达的基因 还将确定以开始检查SRFA在 芽孢杆菌发育。