PEPTIDE SECONDARY METABOLITE BIOSYNTHESIS IN BACILLUS

芽孢杆菌中肽次级代谢产物的生物合成

• 批准号: 2183502
• 负责人: PETER ZUBER
• 金额: $ 12.12万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2183502
• 关键词: Bacillus; Escherichia coli; antibiotics; bacterial genetics; developmental genetics; gene expression; genetic manipulation; genetic mapping; genetic promoter element; genetic transcription; growth media; mutant; nucleic acid sequence; operon; peptides; protein biosynthesis; surfactant; transposon /insertion element

Unicellular organisms as well as the cells of higher organisms utilize low molecular weight polypeptides as extracellular signals that regulate cell differentiation and specialization. Spore- forming bacteria of the genus Bacillus are known to produce a number of peptides which possess antimicrobial activity. More recently, evidence has emerged which implicates these peptides as regulators of sporulation and competence development. Surfactin is a cyclic lipopeptide and powerful biosurfactant produced by B. subtilis. Genes required for surfactin production have been identified and isolated. These include the srfA operon, which encodes at least one of the enzymes involved in the synthesis of surfactin. Genetic evidence has shown that srfA is also required for competence development and efficient sporulation, which suggests that peptides synthesized by the srfA enzymes may function in developmental processes. Transcription of srfA is activated in stationary phase cells and is repressed by readily metabolized carbon and nitrogen sources. Production of surfactin also requires the comA gene, a putative transcriptional regulator of competence gene and srfA transcription. The sfp gene is also required for surfactin production, but its primary function of is unknown. The goals of the experiments described herein is to identify and characterize the products of the srfA and sfp genes, and to elucidate the mechanisms of srfA transcriptional regulation. Genes that require srfA for their expression will also be identified in order to begin to examine the role of srfA in Bacillus development.

单细胞生物以及高等生物的细胞利用低 分子量多肽作为细胞外信号调节细胞 差异化和专业化。 产孢子细菌属 已知芽孢杆菌产生许多肽，其具有 抗菌活性 最近，有证据表明， 暗示这些肽作为孢子形成和感受态的调节剂 发展 表面活性素是一种环状脂肽，是一种功能强大的生物表面活性剂 由B生产。枯草杆菌。 表面活性素产生所需的基因已经被 识别和隔离。这些包括srfA操纵子，其编码在 参与表面活性素合成的酶中的至少一种。 遗传 有证据表明，能力发展也需要自我反思 和有效的孢子形成，这表明，肽合成的 srfA酶可能在发育过程中起作用。 转录 srfA在稳定期细胞中被激活，并容易被 代谢的碳源和氮源。 表面活性素的生产还 需要comA基因，一个假定的转录调控能力 基因和srfA转录。 sfp基因也是表面活性素所必需的 生产，但其主要功能是未知的。 的目标 本文所述的实验是鉴定和表征产物 的srfA和sfp基因，并阐明srfA的机制， 转录调控 需要srfA表达的基因 为了开始研究srfA在以下方面的作用， 芽孢杆菌发育。