MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
批准号:
2182952
负责人:
PETER D MOORE
金额:
$18.24万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-01-01 至 1995-12-31
关键词:
alleles binding proteins cell cycle eukaryote gene mutation gene therapy genetic crossing over genetic manipulation genetic recombination genotype heterozygote homozygote hypoxanthine phosphoribosyltransferase molecular cloning nucleic acid sequence plasmids point mutation protein purification recombinant DNA tissue /cell culture
中文摘要
哺乳动物同源重组机制的探讨
英文摘要
An understanding of the mechanisms of homologous recombination in mammalian
cells is of importance both for its general contribution to our knowledge
of genetic processes and for its practical application in accomplishing
gene targeting. Gene targeting will play a major role in analyzing the
function of cloned genes by mutational analysis and will in the long term
contribute to the development of gene therapy procedures. It has long been
understood that specific sequences may exist that promote homologous
recombination, and in recent years circumstantial evidence that the
hypervariable minisatellite loci, ubiquitous in higher eukaryotes, may play
such a role has been accumulating. We have recently demonstrated that a
synthetic sequence SAT, showing homology with natural minisatellites is a
hot spot for recombination between plasmids in human cells. The goals of
this proposal are to investigate the mechanism of recombination stimulation
by SAT and natural minisatellites, to analyse their role in other cellular
recombination events and to exploit these sequences to improve gene
targeting methodologies. It is intended to introduce SAT sequences into an
established gene targeting system to determine whether and how
minisatellites may affect this process. The possible involvement of
minisatellites in the process of sister chromatid exchange will be examined
by using appropriate probes to investigate the recognized process of new
length allele formation in minisatellite loci to determine whether this
occurs by unequal recombination events and whether these preferentially
occur at these loci. Several approaches to understanding the mechanism of
recombination stimulation by minisatellites will be undertaken. These
include the purification and characterization of a protein shown to
specifically bind to the SAT sequence, an extensive analysis of the
products of recombination and analysis of the affects of distance and
heterology between the minisatellites and the recombination events and of
the number of minisatellite repeats present in the substrates.
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MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
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批准号:2182953
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项目类别:
-
资助金额:$18.73万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
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批准号:3304487
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项目类别:
-
资助金额:$14.93万
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财政年份:1991
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负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
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批准号:3304490
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项目类别:
-
资助金额:$17.2万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
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批准号:3304489
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项目类别:
-
资助金额:$17.7万
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财政年份:1991
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负责人:PETER D MOORE
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依托单位:
REPLICATION OF DAMAGED DNA IN MAMMALIAN CELL EXTRACTS
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批准号:3174875
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项目类别:
-
资助金额:$16.93万
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财政年份:1983
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负责人:PETER D MOORE
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依托单位:
REPLICATION OF DAMAGED DNA IN MAMMALIAN CELL EXTRACTS
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批准号:3174876
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项目类别:
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资助金额:$13.51万
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财政年份:1983
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负责人:PETER D MOORE
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依托单位:
海外基金