REPLICATION OF DAMAGED DNA IN MAMMALIAN CELL EXTRACTS
REPLICATION OF DAMAGED DNA IN MAMMALIAN CELL EXTRACTS
批准号:
3174876
负责人:
PETER D MOORE
金额:
$13.51万
依托单位国家:
美国
项目类别:
财政年份:
1983
资助国家:
美国
项目状态:
已结题
起止时间:
1983-09-01 至 1988-02-28
中文摘要
DNA损伤的大多数遗传效应是在DNA过程中发生的
英文摘要
Most of the genetic effects of DNA damage are effected during DNA
replication. Lesions in the DNA block replication: subsequently, however,
repair mechanisms allow the blocks to be circumvented. One of the major
post-replication repair pathways in mammalian cells occurs by de novo
synthesis. This pathway may be related to the inducible process of repair
and mutagenesis observed with several viruses. We propose to examine
replication as carried out in vitro by extracts from mouse and Chinese
Hamster cells of DNA damaged by UV and N-acetoxy-AAF, analyzing the
products at the level of the DNA sequence. We will compare synthesis in
extracts from untreated cells with those from cells pre-treated with UV
irradiation to examine any inducible changes in synthesization damaged
DNA. Using 0X174 and paravovirus DNA as templates we will locate the
precise sites of blocks, quantitate termination and investigate the ability
to carry out trans-lesion synthesis. We will examine the parameters that
lead to termination at or by-pass of lesions, particularly in relation to
pre-treatment of cells with UV. Any trans-lesion synthesis will be
examined as to its efficiency and the specificity of nucleotide insertion
at the sites of damage. This experimental strategy will provide a bridge
between previous in vivo approaches and in vitro experiments with purified
polymerases in order to further our understanding of the processes involved
in the replication of mutagen damaged DNA.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.
酿酒酵母 rad 和 rnc 突变体的体外重组。
DOI:
10.1007/bf00336741
发表时间:
1993
期刊:
Current genetics
影响因子:
2.5
作者:
[Moore,PD, Simon,JR, Wallace,LJ, Chow,TY]
通讯作者:
Chow,TY
DOI:
10.1007/bf00265060
发表时间:
1990
期刊:
Molecular & general genetics : MGG
影响因子:
--
作者:
[Simon,JR, Moore,PD]
通讯作者:
Moore,PD
Homologous recombination in a Chinese hamster X-ray-sensitive mutant.
中国仓鼠 X 射线敏感突变体的同源重组。
DOI:
10.1016/0027-5107(86)90038-2
发表时间:
1986
期刊:
Mutation research
影响因子:
--
作者:
[Moore,PD, Song,KY, Chekuri,L, Wallace,L, Kucherlapati,RS]
通讯作者:
Kucherlapati,RS
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
-
批准号:2182952
-
项目类别:
-
资助金额:$18.24万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
-
批准号:2182953
-
项目类别:
-
资助金额:$18.73万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
-
批准号:3304487
-
项目类别:
-
资助金额:$14.93万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
-
批准号:3304489
-
项目类别:
-
资助金额:$17.7万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
MINISATELLITE SEQUENCES AND HOMOLOGOUS RECOMBINATION
-
批准号:3304490
-
项目类别:
-
资助金额:$17.2万
-
财政年份:1991
-
负责人:PETER D MOORE
-
依托单位:
REPLICATION OF DAMAGED DNA IN MAMMALIAN CELL EXTRACTS
-
批准号:3174875
-
项目类别:
-
资助金额:$16.93万
-
财政年份:1983
-
负责人:PETER D MOORE
-
依托单位:
海外基金