SENSITIVE AND SELECTIVE DETECTION OF PEPTIDES

拟议工作的长期目标是为研究人员和 有能力测定多肽激素的临床研究人员 和超微量水平的神经递质 生物材料。特别是，将开发一个系统，该系统具有 对其通用性至关重要的几个独特属性。这个 用作高压液相色谱检测器的系统 (高效液相色谱)，将检测到任何多肽。它将在这样的选择性下运行 只有多肽才会给出信号，检测下限为1 用于普通高效液相色谱的飞托莫尔有望实现。新生物活性物质的发现 多肽，多肽激素分析的改进，加深对 多肽在器官和系统中的作用以及对蛋白质的改进 微测序和更好的药物分析将是直接的结果 这部作品的价值。 缩二脲反应，长期以来被认为是蛋白质方法的基础 决心，对多肽有效。缩二脲络合物是可氧化的，所以 多肽可以在阳极上通过电化学方法检测到，因为它们是缩二脲 复合体，在它们被分离为天然多肽后。这是 使提出的氧化体系不稳定的基础化学 缩二脲复合体。氧化缩二脲络合物可在阴极处检测到 在阳极的下游。阴极上的电流信号将是一个 如果氧化的缩二脲络合物暴露在切碎的 光源。低检测下限将是一个结果，在一定程度上 选择性好，部分以精心匹配的微电极阵列为主 从理论上用测量电子学进行检测。

The long range objective of the work proposed is to provide researchers and clinical investigators with the capability to determine peptide hormones and neurotransmitters at ultra-trace levels in very small quantities of biological materials. In particular, a system will be developed that has several unique attributes which are critical to its generality. The system, meant for use as a detector for high pressure liquid chromatography (hplc), will detect any peptide. It will operate with such selectivity that only peptides will give a signal, and detection limits of one femtomole for ordinary hplc are expected. The discovery of new bioactive peptides, improvement in peptide hormone assays, deeper understanding of the roles of peptides in organs and systems, improvements in protein microsequencing and better pharmaceutical analysis will be a direct result of this work. The biuret reaction, long known as the basis of a method for protein determination, works for peptides. The biuret complex is oxidizable, so peptides can be detected electrochemically at an anode, as their biuret complexes, following their separation as native peptides. This is the basic chemistry that makes the proposed system instability of the oxidized biuret complex. The oxidized biuret complex can be detected at a cathode downstream of the anode. The current signal at the cathode will be an alternating one if the oxidized biuret complex is exposed to a chopped light source. The low detection limits will be a result, in part of the selectivity, and in part of carefully matching microelectrode array-based detection with the measurement electronics according to theory.