CELL-CELL SIGNALING AND COMPETENCE IN BACILLUS SUBTILIS
CELL-CELL SIGNALING AND COMPETENCE IN BACILLUS SUBTILIS
批准号:
2189065
负责人:
ALAN D GROSSMAN
金额:
$23.73万
依托单位国家:
美国
项目类别:
财政年份:
1994
资助国家:
美国
项目状态:
已结题
起止时间:
1994-05-01 至 1998-04-30
中文摘要
细胞间信号传导控制着生物学中的许多过程,
世界,包括发展,发病机制,生长,交配,
转型信号传导过程通常由以下因素介导:
(e.g.激素、信息素、神经递质),
一些细胞被其他细胞感知为了了解监管
涉及细胞间信号传导的过程,识别
信号分子,决定它们是如何产生的,
感知,并确定信号是如何转导的,
监管回应。
这个项目的长期目标是了解细胞-细胞
信号传导控制芽孢杆菌中的基因表达和分化
枯草杆菌。B。枯草杆菌产生胞外信号分子
其至少部分是在培养物中积累的寡肽
当细胞生长到高密度时,这些细胞外信号
肽刺激发育所需基因的表达
基因能力。B中的遗传能力。subtilis是
被外源DNA转化的天然能力,
基因交换的有效途径。我们的目标是净化和
从生物化学上表征诱导
感受态基因表达和感受态,以表征如何
产生这些细胞外信号分子,
表征细胞感知和响应的机制
这些肽因子,并确定信号因子是否
参与能力也影响孢子形成。
这些目标包括描述幽灵的角色
(opp)操纵子在细胞对肽信号传导因子的反应中。
spoOK的突变导致能力(和孢子形成)缺陷
阻止细胞对肽信号的反应
刺激感受态基因表达的分子。假脱机编码
一种寡肽通透酶,其是以下大家族的成员:
ATP依赖性转运蛋白。这个运输系统家族
包括MDR,涉及多药耐药,CFTR,囊性
纤维化跨膜调节因子和溶血素所需的Hly
输出某些致病菌。也包括在这个家庭的
转运蛋白是来自细菌的多种渗透酶,
吸收许多化合物,包括组氨酸,麦芽糖,磷酸盐,
二肽、维生素B 12和铁。其中一个重要目的是
建议是确定如何编码的寡肽渗透酶
吓到B了枯草芽孢杆菌参与控制
能力基因我们对分化的研究,
简单、实验上可获得的微生物
细胞间信号传导的一般机制,
转导和调节基因表达,
分化
英文摘要
Cell-cell signaling controls many processes in the biological
world, including development, pathogenesis, growth, mating, and
transformation. Signaling processes are often mediated by factors
(e.g. hormones, pheromones, neurotransmitters) that are produced by
some cells and sensed by others. In order to understand regulatory
process involving cell-cell signaling, it is essential to identify
the signaling molecules, determine how they are produced and
sensed, and determine how the signals are transduced to elicit a
regulatory response.
The long term goal of this project is to understand how cell-cell
signaling controls gene expression and differentiation in Bacillus
subtilis. B. subtilis produces extracellular signaling molecules
that are at least in part oligopeptides that accumulate in culture
medium as cells grow to high density. These extracellular signaling
peptides stimulate expression of genes needed for the development
of genetic competence. Genetic competence in B. subtilis is the
natural ability to be transformed by exogenous DNA and is an
efficient means of genetic exchange. Our aims are to purify and
biochemically characterize the signaling molecules that induce
competence gene expression and competence, to characterize how
these extracellular signaling molecules are produced, to
characterize the mechanisms by which cells sense and respond to
these peptide factors, and to determine if the signaling factors
involved in competence also affect sporulation.
Included in these aims is to characterize the role of the spoOK
(opp) operon in the cellular response to peptide signaling factors.
Mutations in spoOK cause a defect in competence (and sporulation)
and prevent cells from responding to the peptide signaling
molecules that stimulate competence gene expression. spoOK encodes
an oligopeptide permease that is a member of the large family of
ATP-dependent transporters. This family of transport systems
includes MDR, involved in multi-drug resistance, CFTR, the cystic
fibrosis transmembrane regulator, and Hly, required for hemolysin
export in some pathogenic bacteria. Also included in this family of
transporters are a variety of permeases from bacteria that mediate
uptake of many compounds, including histidine, maltose, phosphate,
dipeptides, vitamin B 12, and iron. An important aim of this
proposal is to determine how the oligopeptide Permease encoded by
spoOK of B. subtilis is involved in controlling expression of
competence genes. Our studies on differentiation in this relatively
simple, experimentally accessible microbe should provide insights
into general mechanisms of cell-cell signaling, signal
transduction, and regulation of gene expression and
differentiation.
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资助金额:$40.23万
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批准号:6180251
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资助金额:$31.11万
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资助金额:$40.47万
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依托单位:
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资助金额:$38.42万
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资助金额:$38.42万
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批准号:6935371
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批准号:2415238
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批准号:2189067
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项目类别:
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资助金额:$25.13万
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依托单位:
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项目类别:
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资助金额:$40.43万
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-
依托单位:
海外基金