INTRACELLULAR OPTICAL NANOSENSORS--PH, CALCIUM, GLUCOSE

细胞内光学纳米传感器--PH、钙、葡萄糖

• 批准号: 2188037
• 负责人: Raoul Kopelman
• 金额: $ 20.2万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2188037
• 关键词: acidity /alkalinity; alternatives to animals in research; biomedical equipment development; biosensor device; calcium flux; fiber optic microscopy; fiber optics; fluorescent dye /probe; glucose transport; intracellular transport; microelectrodes; monitoring device; respiratory gas transport

The objective is to produce nano-fiberoptic chemical sensors for the direct, real-time monitoring of intact single cell processes. These sensors will monitor concentrations of analytes such as glucose, calcium, oxygen, and hydrogen ions, as well as their sub-microscopic chemical maps and time developments, individually or simultaneously. The samples required for these sensor diameters will be 100-1000 times smaller and their response times 100-1000 times shorter. They will also be faster, less invasive and more selective than comparable electrochemical sensors. The aim is to apply recent theoretical and technological breakthroughs: 10 The construction of subwavelength optical light sources and sensor down to sizes of 40 nm. 2) The demonstration that near-field optics increases the molecular cross-section for optional absorption up to a million times. The initial step is a rugged pH fiber-optic sensor with millisecond response time and excellent sensitivity, requiring only atto- liters (10-181) of sample. Specifically, the proposal outlines how to design and carry out appropriate dye sensor fabrication schemes as well as how to construct and test such ultra-small and ultra-fast model sensors for intracellular measurements of calcium, oxygen and glucose, in addition to pH. These will also be working models for generalize ion sensors and for drug sensors. We also design a method for micron and submicron chemical mapping, combined with optical imaging. This includes scanning near-field optical imaging and sensing down to a spatial resolution of 0,05 microns. Such nano-chemical sensors technology will speed up by a large factor various biochemical test protocols and might well replace existing animal testing protocols and might well replace existing animal testing protocols by much less costly and sager single cell or single embryo protocols. These tests and protocols will be on single rat live cells, blood cells etc. and on early rat embryos. The long range goal is to make optical nanosensors with single molecule/ion spatial resolution and chemical sensitivity.

目的是生产纳米纤维化化学传感器 直接的，实时监视完整的单细胞过程。 这些 传感器将监视分析物的浓度，例如葡萄糖，钙， 氧气和氢离子及其亚显微镜化学图 和时间发展，单独或同时。 样品 这些传感器直径所需的需要小100-1000倍，并且 他们的响应时间短100-1000倍。 他们也会更快 与可比的电化学传感器相比，侵入性更少，更有选择性。 目的是应用最新的理论和技术突破： 10次​​波长光源和传感器的构建 下降到40 nm的尺寸。 2）近场光学的演示 增加分子横截面以使可选吸收到A 百万次。 最初的步骤是一个坚固的pH纤维传感器 毫秒的响应时间和出色的灵敏度，仅需要 样品的升（10-181）。 具体而言，该提案概述了如何 设计并执行适当的染料传感器制造方案 就像如何构建和测试此类超小型和超快速模型 钙，氧和葡萄糖的细胞内测量的传感器， 除了pH。 这些也将是概括离子的工作模型 传感器和药物传感器。 我们还为微米设计了一种方法 亚微米化学映射，结合光学成像。 这包括 扫描近场光学成像并传感到空间 分辨率为0,05微米。 这样的纳米化学传感器技术将 通过各种生化测试方案加快速度 很好地替换现有的动物测试方案，很可能会取代 现有的动物测试协议的成本较低，而Sager单一 单元或单个胚胎协议。 这些测试和协议将开始 单大鼠活细胞，血细胞等以及早期大鼠胚胎。 这 远距离目标是用单分子/离子制作光学纳米传感器 空间分辨率和化学敏感性。