POSTPRANDIAL LIPID METABOLISM IN CORONARY ARTERY DISEASE

冠状动脉疾病的餐后脂质代谢

• 批准号: 5213123
• 负责人: CHRISTOPHER J FIELDING
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5213123
• 关键词: apolipoproteins; atherosclerosis; blood lipoprotein metabolism; cardiovascular disorder diagnosis; cholesterol; chylomicrons; clearance rate; coronary disorder; dietary lipid; high density lipoproteins; human subject; hypercholesterolemia; lipid metabolism; lipoprotein disorder; low density lipoprotein; monoclonal antibody; nutrition related tag; pathologic process; retinoids; steroid metabolism; triglycerides

Fasting and postprandial lipid metabolism will be studied in the plasma of 200 men and women (50-69 years of age) with angiographically documented coronary artery disease (CAD) but with normal (<90th percentile) LDL-cholesterol and plasma triglyceride levels. The data obtained will be compared with those in a normal control group without clinical evidence of CAD, who have a negative stress test. Following a dietary challenge of whole foods supplemented with vitamin A, the removal of chylomicron apo B-48 protein will be followed postprandially, using a new monoclonal antibody method. Over the same time period, circulating levels of retinyl ester, high and low density lipoprotein (HDL and LDL) subfractions, and other metabolic factors will be assayed in order to test three different hypotheses relating to human atherogenesis, In the first, we will test whether chylomicron remnant clearance is delayed, and whether retinyl ester is a marker for apo B-48, in this group of CAD patients. In the second, we will test the hypothesis that plasma cholesterol metabolism is abnormal in CAD and in particular, that reverse cholesterol transport is reduced. In the third, we will test whether an unusual pattern of abnormal HDL and LDL subfractions, characterized by increased prebeta-HDL and/or lipid-poor LDL, is diagnostic for these patients. Together, these studies can be expected to provide new information on an important and little-studied group of men and women with CAD, and have the potential to provide important insights into the mechanism of human atherogenesis in general.

空腹和餐后脂代谢将在血浆中进行研究 对200名男性和女性(50-69岁)进行了血管造影 有记录的冠状动脉疾病(CAD)，但正常(&lt；第90 百分位数)--低密度脂蛋白-胆固醇和血浆甘油三酯水平。数据 将获得的结果与正常对照组的结果进行比较 冠心病的临床证据，他们的压力测试呈阴性。在此之后 补充维生素A的全食的饮食挑战，去除 乳杆菌载脂蛋白B-48蛋白的研究将在餐后进行，使用 一种新的单抗方法。在同一时间段内，循环 视黄酯、高密度脂蛋白和低密度脂蛋白(高密度脂蛋白和低密度脂蛋白) 亚组分和其他代谢因素将被检测，以便 测试与人类动脉粥样硬化形成有关的三种不同假说 首先，我们将测试乳杆菌残留物是否被延迟清除，以及 在这组CAD中，视黄酸酯是否为载脂蛋白B-48的标志物 病人。在第二个实验中，我们将检验等离子体的假设 冠心病患者的胆固醇代谢异常，尤其是相反 胆固醇的运输减少了。在第三个测试中，我们将测试一个 异常的高密度脂蛋白和低密度脂蛋白亚组分的异常模式，特征是 升高的前β-高密度脂蛋白和/或低密度脂蛋白是这些疾病的诊断指标 病人。总而言之，这些研究有望提供新的 关于一个重要而鲜为人知的男女群体的信息 ，并有可能提供对 人类动脉粥样硬化形成的一般机制。