DIETARY CHOLESTEROL EFFECTS ON PLASMA LIPOPROTEINS

膳食胆固醇对血浆脂蛋白的影响

• 批准号: 3358848
• 负责人: CHRISTOPHER J FIELDING
• 金额: $ 42.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3358848
• 关键词: apolipoproteins; blood chemistry; blood lipoprotein; blood lipoprotein metabolism; blood lipoprotein transport; cholesterol; dietary lipid; high density lipoproteins; homeostasis; human middle age (35-64); human subject; hyperlipidemia; lipid transport; liver metabolism; low density lipoprotein; male; membrane lipids; nutrition related tag; phosphatidylcholine sterol acyltransferase; saturated fat; young adult human (21-34)

Increased dietary cholesterol and saturated fat have been shown to be related to increased levels of plasma cholesterol (particularly in low density lipoproteins) and vascular disease. The purpose of this study is to determine the effects of dietary cholesterol, in combination with low or high intake of saturated fat, on human plasma cholesterol metabolism. The study will focus particularly on the biochemical reactions in plasma promoting the return of peripheral cell cholesterol to the liver (reverse cholesterol transport). Studies in human subjects and lower primates (babboons) have found such transport to be acutely stimulated in postprandial lipemia. Preliminary data now suggests that this stimulation is markedly inhibited by chronic (6-8 week) increased intake of dietary cholesterol. Young and older male volunteers will consume diets of traditional format differing in cholesterol and saturated fat content, prepared in the General Clinical Research Center. The plasma mass and activities of components of the reverse cholesterol transport pathway will be determined in fasting plasma, and during postprandial lipemia, in these subjects adapted to each diet. These measurements will include not only those made on the levels and compositions of the lipoproteins themselves, but also the enzyme and other catalysts promoting the modification and clearance of lipoprotein cholesterol. The data to be obtained will provide detailed new documentation of the effects of dietary cholesterol on plasma lipoprotein metabolism and in particular, indicate whether dietary cholesterol inhibits the return of peripheral cell cholesterol to the liver in human subjects.

饮食中胆固醇和饱和脂肪的增加已被证明 与血浆胆固醇水平升高有关 （特别是低密度脂蛋白）和血管疾病。 的 这项研究的目的是确定饮食的影响， 胆固醇，结合低或高摄入的饱和 脂肪，对人体血浆胆固醇代谢的影响。 这项研究将 特别关注血浆中的生化反应 促进外周细胞胆固醇返回肝脏 （胆固醇逆向转运）。 人类受试者研究， 低等灵长类动物（狒狒）发现这种运输是急性的 在餐后脂血症中刺激。 初步数据显示 这种刺激被慢性（6-8周） 增加膳食胆固醇的摄入。 年轻和年长男性 志愿者将食用传统形式的饮食， 胆固醇和饱和脂肪的含量，在一般编写 临床研究中心。 血浆质量和活性 胆固醇逆向转运途径的组成部分将是 在空腹血浆中和餐后脂血症期间测定， 这些受试者适应了每种饮食。 这些测量将 不仅包括那些对水平和组成的 脂蛋白本身，还有酶和其他催化剂 促进脂蛋白的修饰和清除 胆固醇 将获得的数据将提供详细的新的 膳食胆固醇对血浆的影响的记录 脂蛋白代谢，特别是表明饮食是否 胆固醇抑制外周细胞胆固醇返回到 人类受试者的肝脏