FETAL TISSUE AND CELL CULTURES FOR RESEARCH

提出了一项可行性研究，以探索各种组织的用途 用于移植和相关研究。 具体来说，流产的胎儿加上父母的血液样本和所有相关的 临床数据将通过当地一家医院综合体获得。什么时候都行 可能，胰腺、皮肤和肌肉、肝脏、肾脏、软骨和肺 组织将被取出，分离，并细分为 超低温保存和体外培养试验。现有的协议用于 这些操作将根据需要进行比较和改进。 克隆培养、细胞团维持技术及饲养器的应用 细胞群体将在适当的地方被利用来发展 定量比较数据。组织学和生化分析将是 既用于评估分离/培养方法，也用于鉴定 维持功能细胞的最佳培养条件。细胞 将根据表面人类白细胞抗原的表达来确定人群的特征 和其他重要的移植抗原，并将配置文件 与父母捐赠者的情况相比。组织与细胞培养 将对人群进行细菌、真菌、 支原体、HIV、CMV、乙肝等病毒。细胞遗传学分析 将确认正常的二倍体状态。将制作相关的细胞库 可用于促进与其他机构的合作。

A feasibility study is proposed to explore the utility of various tissues from human abortuses for transplantation and related research. Specifically, aborted fetuses plus parental blood samples and all relevant clinical data will be obtained through a local hospital complex. Whenever possible, pancreas, skin and muscle, liver, kidney, cartilage and lung tissues will be removed, dissociated and subfractionated for cryopreservation and cultivation trials in vitro. Existing protocols for these manipulations will be compared and improved upon as required. Clonal culture, cell aggregate maintenance techniques and use of feeder cell populations will be utilized where appropriate to develop quantitative comparative data. Histological and biochemical assays will be applied both to evaluate separation/cultivation methods and to identify optimal culture conditions for maintaining functional cells. Cell populations will be characterized with regard to expression of surface HLA and other significant transplantation antigens, and profiles will be compared to those of the parental donors. Tissue and cell culture populations will be examined for infections with bacteria, fungi, mycoplasma, HIV, CMV, hepatitis B and other viruses. Cytogenetic analyses will confirm the normal diploid status. Pertinent cell stocks will be made available to foster collaboration with other institutions.