FETAL TISSUE AND CELL CULTURES FOR RESEARCH

用于研究的胎儿组织和细胞培养

• 批准号: 2205169
• 负责人: ROBERT J HAY
• 金额: $ 24.25万
• 依托单位: AMERICAN TYPE CULTURE COLLECTION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-23 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2205169
• 关键词: SDS polyacrylamide gel electrophoresis; affinity chromatography; cell transplantation; cryopreservation; cytogenetics; histocompatibility antigens; histocompatibility typing; human fetus tissue; human subject; induced abortion; polymerase chain reaction; tissue /cell culture; tissue resource /registry; transplantation immunology; western blottings

A feasibility study is proposed to explore the utility of various tissues from human abortuses for transplantation and related research. Specifically, aborted fetuses plus parental blood samples and all relevant clinical data will be obtained through a local hospital complex. Whenever possible, pancreas, skin and muscle, liver, kidney, cartilage and lung tissues will be removed, dissociated and subfractionated for cryopreservation and cultivation trials in vitro. Existing protocols for these manipulations will be compared and improved upon as required. Clonal culture, cell aggregate maintenance techniques and use of feeder cell populations will be utilized where appropriate to develop quantitative comparative data. Histological and biochemical assays will be applied both to evaluate separation/cultivation methods and to identify optimal culture conditions for maintaining functional cells. Cell populations will be characterized with regard to expression of surface HLA and other significant transplantation antigens, and profiles will be compared to those of the parental donors. Tissue and cell culture populations will be examined for infections with bacteria, fungi, mycoplasma, HIV, CMV, hepatitis B and other viruses. Cytogenetic analyses will confirm the normal diploid status. Pertinent cell stocks will be made available to foster collaboration with other institutions.

建议进行可行性研究，以探索各种组织的效用 用于移植和相关研究。 特别是，流产胎儿加上父母的血液样本和所有相关的 临床数据将通过当地医院综合体获得。 每当 可能，胰腺、皮肤和肌肉、肝脏、肾脏、软骨和肺 组织将被移除、分离和亚分级， 冷冻保存和体外培养试验。 现有协议 这些操作将根据需要进行比较和改进。 克隆培养、细胞聚集体维持技术和饲养层的使用 细胞群将在适当的情况下用于开发 定量比较数据。将进行组织学和生物化学测定。 应用于评估分离/培养方法和鉴定 维持功能细胞的最佳培养条件。 细胞 群体将根据表面HLA的表达来表征 和其他重要的移植抗原，和概况将是 与父母捐赠者相比。 组织和细胞培养 将对人群进行细菌，真菌， 支原体、HIV、CMV、B型肝炎和其它病毒。细胞遗传学分析 将确认正常的二倍体状态将制作相关的细胞储备 以促进与其他机构的合作。