NATIONAL STEM CELL RESOURCE

国家干细胞资源

• 批准号: 6529882
• 负责人: ROBERT J HAY
• 金额: $ 56.79万
• 依托单位: AMERICAN TYPE CULTURE COLLECTION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529882
• 关键词: animal tissue; cell bank /registry; embryonic stem cell; genotype; molecular biology information system; sample collection; stem cells

Embryonal stem (ES) cells and lineage- or tissue-specific stem cells are important models in biomedical studies, but the availability and accessibility of research materials in this rapidly advancing field often become limiting. These restrictions can be addressed by a central, internationally recognized Stem Cell Resource devoted to acquisition and distribution of cells, reagents and information on nonhuman ES cells and postnatally derived stem cells from a variety of species. ATCC is the oldest, largest and most experienced organization engaging in this type of endeavor. Mechanisms for efficient and cost-effective accessioning, characterization, expansion, preservation and distribution of biomaterials and information are firmly in place and highly utilized daily. To a large degree these procedures were developed at ATCC out of necessity, providing an atmosphere fostering continued experimentation and improvement. In addition, several ATCC scientists are pursuing research relevant to stem cell biology, providing a critical research orientation to the Resource. We propose to accession, characterize, expand, preserve and distribute nonhuman ES cells, as well as postnatally derived stem cells from a variety of strains and species. Through a consortium arrangement under the direction of Dr. Barbara Knowles at The Jackson Laboratories, germline competency of murine ES cells will be confirmed in a quantitative manner prior to distribution, - We will also carry out Resource-related research, in partnership with Dr. James Thomson, Univ. of Wisconsin, to extensively characterize stem cells with regard to biological properties and utility of molecular tools used in stem cell research. We will standardize and opting methodology and develop new reagents in an attempt to make procedures and reagents broadly applicable across a range of species and strains. We will also establish interactive, web-based mechanisms to distribute information and technology as well as materials, and serve as a centralized source for information exchange and rapid dissemination of advances in this extremely active field.

胚胎干细胞和谱系或组织特异性干细胞是生物医学研究中的重要模型，但在这一快速发展的领域中研究材料的可用性和可及性往往变得有限。这些限制可以通过一个中央的、国际公认的干细胞资源来解决，该资源致力于获取和分布关于各种物种的非人类ES细胞和出生后来源的干细胞的细胞、试剂和信息。ATCC是从事这类努力的历史最悠久、规模最大、经验最丰富的组织。生物材料和信息的有效和具有成本效益的获取、表征、扩展、保存和分发的机制牢牢到位，每天都得到高度利用。这些程序在很大程度上是ATCC出于需要而开发的，提供了一个促进持续试验和改进的氛围。此外，几位ATCC科学家正在从事与干细胞生物学相关的研究，为该资源提供了一个关键的研究方向。我们建议加入、鉴定、扩增、保存和分配非人类胚胎干细胞，以及来自各种品系和物种的出生后来源的干细胞。通过在杰克逊实验室Barbara Knowles博士的指导下的财团安排，小鼠ES细胞的种系能力将在分配之前以定量的方式得到确认，我们还将与大学詹姆斯·汤姆森博士合作开展资源相关研究。关于干细胞的生物学特性和干细胞研究中使用的分子工具的效用的广泛特征。我们将标准化和选择方法学，并开发新的试剂，试图使程序和试剂广泛适用于一系列物种和菌株。我们还将建立以网络为基础的互动机制，分发信息和技术以及材料，并作为信息交流和迅速传播这一极为活跃领域的进展的集中来源。