PROCUREMENT OF CELLS & DNA FOR GENETIC LINKAGE STUDIES

该项目的目标是提供必要的生物 研究癌症遗传易感性所需的底物。 提供者将启动淋巴细胞的转化与EB病毒 病毒以建立成淋巴细胞的长期培养物。 这些 细胞随后将用于提取DNA用于遗传标记研究。 DNA也将直接从粒细胞和淋巴细胞中提取 在全血中，来自培养的成纤维细胞和肿瘤，以及来自新鲜肿瘤 材料 从这些试验中获得的DNA将用于 环境流行病学分支（家庭研究）的调查人员 科）及临床流行病学分支（临床遗传学组） 正在进行的遗传病因学导致的癌症易感性研究。

The objectives of this project are to provide essential biological substrates required for the study of genetic susceptibility to cancer. The offeror will initiate transformation of lymphocytes with Epstein-Barr virus in order to establish long term cultures of lymphoblasts. These cells will subsequently be used to extract DNA for genetic marker studies. DNA will also be extracted directly from the granulocytes and lymphocytes in whole blood, from cultured fibroblasts and tumors, and from fresh tumor material. The DNAs obtained from these assays will be used by investigators in the Environmental Epidemiology Branch (Family Studies Section) and the Clinical Epidemiology Branch (Clinical Genetics Section) in ongoing studies of cancer susceptibility due to genetic etiology.