HIGH-RESOLUTION EXTENDED DNA MAPPING OF YACS

YACS 的高分辨率扩展 DNA 作图

• 批准号: 2209210
• 负责人: BRAD E. WINDLE
• 金额: $ 12.72万
• 依托单位: CTRC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-12-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2209210
• 关键词: DNA; artificial chromosomes; computer assisted sequence analysis; computer program /software; digital imaging; fluorescence microscopy; fluorescent dye /probe; genetic mapping; genetic techniques; genome; human genetic material tag; in situ hybridization; method development; molecular cloning; nucleic acid probes; nucleic acid structure; polymerase chain reaction

The first goal of this project is to assess the feasibility of using a newly developed, rapid, high resolution, visual mapping procedure for the mapping of yeast artificial chromosomes. The assembly of maps from yeast artificial chromosomes for the human genome will facilitate identification of genetic disorders and the gene responsible. The second goal is to improve this new mapping procedure by developing a multi-color multi-probe strategy and developing computer software capable of analyzing the visual images that are generated. These improvements will allow faster mapping as well as provide the ability to rapidly and precisely detect gene rearrangements involved in human disorders. The new mapping technology that will be used involves the stretching of human genomic DNA on a slide and hybridizing fluorescent DNA probes to the extended DNA strands. The result is a multi-color string as viewed through a fluorescence microscope and represent a map of the DNA region of interest. Resolution as low as .4 Kb has been obtained. Yeast artificial chromosomes from human chromosome 3 will be studied with this technology.

这个项目的第一个目标是评估使用一个