TRAINING IN MOLECULAR VIROLOGY AND AIDS PATHOGENESIS

分子病毒学和艾滋病发病机制培训

• 批准号: 2057607
• 负责人: Anne D Lewis
• 金额: $ 7.32万
• 依托单位: CHILDREN'S HOSPITAL (COLUMBUS)
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2057607
• 关键词: AIDS; AIDS education /prevention; Macaca nemestrina; disease /disorder model; human immunodeficiency virus 1; model design /development; molecular biology; recombinant virus; science education; simian immunodeficiency virus; tissue /cell culture; training

A training program for Dr. Anne Lewis is described in this Physician Scientist Award (K11) application. The program is designed to provide Dr. Lewis with the skills to investigate viral pathogenesis at a molecular level in order for her to ultimately become an independent investigator. Dr. Lewis is a board certified veterinary pathologist who has recently returned to graduate school to pursue a Ph.D. degree in Experimental Pathology in the Department of Veterinary Pathobiology at the Ohio State University. She is co-sponsored by Dr. Philip Johnson and Dr. Paul Stromberg. The specific aims of Phase I of the training program for Dr. Lewis are: (i) to obtain basic training in virology, tissue culture and molecular biology, and (ii) to apply these skills in the construction of a series of molecular chimeras between SIV and HIV-1. Basic training will be accomplished through pertinent didactic coursework, participation in specialized courses covering such topics as advanced in situ hybridization, active benchwork in the laboratory, and regular consultation and interaction with her co-sponsors. The goal of the research proposed for Phase I is the development of a model of HIV-1 infection in non-human primates using SIV/HlV-1 molecular chimera. During the pre-Phase I portion of her training, Dr. Lewis has successfully constructed an SIV/HlV-1 prototype chimera (SHIV) composed of a background SIV with high replicative ability in which the envelope gene is provided by HIV-1. In vitro viability and infectivity for pig-tailed macaques has been demonstrated. The goal of the construction of further chimeras in Phase I is to increase the replicative ability of the chimera by restoring the HIV-1 vpu open reading frame and restoring the SIV tat reading frame and the associated splice junction. Phase I will last 2 years. Completion of Phase I will be determined by completion of Phase I research as described above as well as the successful completion of the requirements for the Ph.D. degree as decreed by the Ohio State University. The specific aims for Phase II research are: (i) to construct SHIV chimeras with divergent HIV-1 envelope genes; (ii) to construct SHIV chimeras with an HIV-1 reverse transcriptase; and (iii) to test additional SHIV chimeras in the pig-tailed macaque model. Phase II is an expansion of skills and ideas developed during Phase I and will last 2 years.

这位医生描述了安妮·刘易斯博士的培训计划。 科学家奖(K11)申请。该计划旨在为Dr。 刘易斯拥有在分子水平上研究病毒发病机制的技能 为了让她最终成为一名独立的调查员，她需要更高的级别。 刘易斯博士是一名委员会认证的兽医病理学家，最近 回到研究生院攻读实验博士学位 俄亥俄州立大学兽医病理生物学系的病理学 大学。她是由菲利普·约翰逊博士和保罗博士共同赞助的 斯特龙伯格。 刘易斯博士培训计划第一阶段的具体目标是： (I)接受病毒学、组织培养和分子方面的基本培训 生物学，以及(Ii)将这些技能应用于构建一系列 SIV和HIV-1之间的分子嵌合体。基本培训将是 通过有针对性的教学课程完成，参与 专业课程涵盖了高级现场培训等主题 杂交、实验室中的活动钳工和常规 与她的共同赞助者进行协商和互动。的目标是 建议进行的第一阶段研究是开发艾滋病毒-1模型 使用SIV/HLV-1分子嵌合体感染非人类灵长类动物。在.期间 在她的培训的第一阶段之前，刘易斯博士成功地 构建了由背景组成的SIV/HLV-1嵌合体(SHIV)原型 具有高复制能力的SIV，其中提供了包膜基因 被HIV-1感染。猪尾猕猴的体外存活率和感染性 已经被证明了。构建更多嵌合体的目标是 第一阶段是通过恢复嵌合体的复制能力 HIV-1 VPU开放阅读框与SIV TAT阅读框的修复 以及相关联的拼接接头。第一阶段将持续两年。完成 将在完成第一阶段研究后确定为 以及需求的成功完成 获得俄亥俄州立大学颁发的博士学位。 第二阶段研究的具体目标是：(I)构建SHIV HIV-1囊膜基因不同嵌合体；(Ii)构建SHIV 带有HIV-1逆转录酶的嵌合体；以及(Iii)测试其他 希夫在猪尾猕猴模型中的嵌合体。第二阶段是 技能和想法是在第一阶段发展起来的，将持续两年。