TRAINING IN MOLECULAR VIROLOGY AND AIDS PATHOGENESIS

分子病毒学和艾滋病发病机制培训

• 批准号: 2671375
• 负责人: Anne D Lewis
• 金额: $ 8.4万
• 依托单位: CHILDREN'S HOSPITAL (COLUMBUS)
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2671375
• 关键词: AIDS; AIDS education /prevention; Macaca nemestrina; disease /disorder model; human immunodeficiency virus 1; model design /development; molecular biology; recombinant virus; science education; simian immunodeficiency virus; tissue /cell culture; training

A training program for Dr. Anne Lewis is described in this Physician Scientist Award (K11) application. The program is designed to provide Dr. Lewis with the skills to investigate viral pathogenesis at a molecular level in order for her to ultimately become an independent investigator. Dr. Lewis is a board certified veterinary pathologist who has recently returned to graduate school to pursue a Ph.D. degree in Experimental Pathology in the Department of Veterinary Pathobiology at the Ohio State University. She is co-sponsored by Dr. Philip Johnson and Dr. Paul Stromberg. The specific aims of Phase I of the training program for Dr. Lewis are: (i) to obtain basic training in virology, tissue culture and molecular biology, and (ii) to apply these skills in the construction of a series of molecular chimeras between SIV and HIV-1. Basic training will be accomplished through pertinent didactic coursework, participation in specialized courses covering such topics as advanced in situ hybridization, active benchwork in the laboratory, and regular consultation and interaction with her co-sponsors. The goal of the research proposed for Phase I is the development of a model of HIV-1 infection in non-human primates using SIV/HlV-1 molecular chimera. During the pre-Phase I portion of her training, Dr. Lewis has successfully constructed an SIV/HlV-1 prototype chimera (SHIV) composed of a background SIV with high replicative ability in which the envelope gene is provided by HIV-1. In vitro viability and infectivity for pig-tailed macaques has been demonstrated. The goal of the construction of further chimeras in Phase I is to increase the replicative ability of the chimera by restoring the HIV-1 vpu open reading frame and restoring the SIV tat reading frame and the associated splice junction. Phase I will last 2 years. Completion of Phase I will be determined by completion of Phase I research as described above as well as the successful completion of the requirements for the Ph.D. degree as decreed by the Ohio State University. The specific aims for Phase II research are: (i) to construct SHIV chimeras with divergent HIV-1 envelope genes; (ii) to construct SHIV chimeras with an HIV-1 reverse transcriptase; and (iii) to test additional SHIV chimeras in the pig-tailed macaque model. Phase II is an expansion of skills and ideas developed during Phase I and will last 2 years.

本《医师》中描述了Anne刘易斯博士的培训计划 科学家奖（K11）申请。该计划旨在提供博士。 刘易斯的技能，研究病毒的发病机制，在分子 最终，她成为了一名独立调查员。 博士刘易斯是一个委员会认证的兽医病理学家谁最近 回到研究生院攻读博士学位。实验学位 俄亥俄州兽医病理学系病理学 大学她由菲利普约翰逊博士和保罗博士共同赞助 斯特龙伯格 刘易斯博士培训计划第一阶段的具体目标是： (i)获得病毒学、组织培养和分子生物学方面的基本培训， 生物学，以及（ii）应用这些技能构建一系列 SIV和HIV-1之间的分子嵌合体。基本训练将是 通过相关的教学课程完成，参与 专门课程，包括高级就地培训、 杂交，实验室中的活跃的钳工工作，以及定期的 与共同赞助者的协商和互动。的目标 第一阶段的研究计划是开发一种HIV-1的模型 使用SIV/HIV-1分子嵌合体在非人灵长类动物中的感染。期间 刘易斯博士在第一阶段训练前， 构建了一个SIV/HIV-1原型嵌合体（SHIV）， 具有高复制能力的SIV，其中提供了包膜基因 HIV-1。猪尾猕猴的体外存活力和感染力 被证明。构建进一步嵌合体的目标是， 第一阶段是通过恢复 HIV-1 vpu打开阅读框并恢复SIV达特阅读框 以及相关的拼接点。第一阶段将持续两年。完成 第一阶段研究的完成情况将决定第一阶段的 上述以及成功完成的要求 对于博士由俄亥俄州州立大学颁发的学位。 第二阶段研究的具体目标是：（i）构建SHIV 嵌合体与不同的HIV-1包膜基因;（ii）构建SHIV 嵌合体与HIV-1逆转录酶;和（iii）测试额外的 猪尾猕猴模型中的SHIV嵌合体。第二阶段是扩大 在第一阶段发展的技能和想法，将持续2年。