TRAINING IN MOLECULAR VIROLOGY AND AIDS PATHOGENESIS

分子病毒学和艾滋病发病机制培训

• 批准号: 2671375
• 负责人: Anne D Lewis
• 金额: $ 8.4万
• 依托单位: CHILDREN'S HOSPITAL (COLUMBUS)
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2671375
• 关键词: AIDS; AIDS education /prevention; Macaca nemestrina; disease /disorder model; human immunodeficiency virus 1; model design /development; molecular biology; recombinant virus; science education; simian immunodeficiency virus; tissue /cell culture; training

A training program for Dr. Anne Lewis is described in this Physician Scientist Award (K11) application. The program is designed to provide Dr. Lewis with the skills to investigate viral pathogenesis at a molecular level in order for her to ultimately become an independent investigator. Dr. Lewis is a board certified veterinary pathologist who has recently returned to graduate school to pursue a Ph.D. degree in Experimental Pathology in the Department of Veterinary Pathobiology at the Ohio State University. She is co-sponsored by Dr. Philip Johnson and Dr. Paul Stromberg. The specific aims of Phase I of the training program for Dr. Lewis are: (i) to obtain basic training in virology, tissue culture and molecular biology, and (ii) to apply these skills in the construction of a series of molecular chimeras between SIV and HIV-1. Basic training will be accomplished through pertinent didactic coursework, participation in specialized courses covering such topics as advanced in situ hybridization, active benchwork in the laboratory, and regular consultation and interaction with her co-sponsors. The goal of the research proposed for Phase I is the development of a model of HIV-1 infection in non-human primates using SIV/HlV-1 molecular chimera. During the pre-Phase I portion of her training, Dr. Lewis has successfully constructed an SIV/HlV-1 prototype chimera (SHIV) composed of a background SIV with high replicative ability in which the envelope gene is provided by HIV-1. In vitro viability and infectivity for pig-tailed macaques has been demonstrated. The goal of the construction of further chimeras in Phase I is to increase the replicative ability of the chimera by restoring the HIV-1 vpu open reading frame and restoring the SIV tat reading frame and the associated splice junction. Phase I will last 2 years. Completion of Phase I will be determined by completion of Phase I research as described above as well as the successful completion of the requirements for the Ph.D. degree as decreed by the Ohio State University. The specific aims for Phase II research are: (i) to construct SHIV chimeras with divergent HIV-1 envelope genes; (ii) to construct SHIV chimeras with an HIV-1 reverse transcriptase; and (iii) to test additional SHIV chimeras in the pig-tailed macaque model. Phase II is an expansion of skills and ideas developed during Phase I and will last 2 years.

这位医生描述了安妮·刘易斯博士的培训计划 科学家奖（K11）申请。该计划旨在提供博士。 刘易斯具有研究分子的病毒发病机制的技能 为了让她最终成为独立调查员。 刘易斯博士是董事会认证的兽医病理学家，最近 回到研究生院攻读博士学位。实验学位 俄亥俄州兽医病理学系的病理学 大学。她由菲利普·约翰逊（Philip Johnson）博士和保罗博士共同赞助。 斯特伦伯格。 刘易斯博士培训计划的第一阶段的具体目的是： （i）获得病毒学，组织培养和分子的基础培训 生物学和（ii）将这些技能应用于一系列 SIV和HIV-1之间的分子嵌合体。基本培训将是 通过相关的教学课程完成，参与 专业课程涵盖了先进的原位主题 杂交，实验室中的主动台式和常规 与她的共同发起人进行咨询和互动。目标的目标 I期提出的研究是HIV-1模型的开发 使用SIV/HLV-1分子嵌合体中非人类灵长类动物的感染。期间 她的培训中的一部分阶段，刘易斯博士成功地 构建了由背景组成的SIV/HLV-1原型嵌合体（SHIV） 具有高复制能力的SIV提供了信封基因 由HIV-1。猪尾猕猴的体外生存力和感染力具有 被证明了。建造进一步的嵌合体的目的 第一阶段是通过还原来提高嵌合体的复制能力 HIV-1 VPU开放阅读框并恢复SIV TAT阅读框 和相关的剪接连接。第一阶段将持续2年。完成 第一阶段的第一阶段研究将确定 上面描述的以及成功完成要求 对于博士学位俄亥俄州立大学颁布的学位。 第二阶段研究的具体目的是：（i）构建SHIV 带有不同HIV-1包膜基因的嵌合体； （ii）构建湿婆 带有HIV-1逆转录酶的嵌合体； （iii）测试其他 猪尾猕猴模型中的湿夫嵌合体。第二阶段是 在第一阶段发展的技能和想法将持续2年。