STRUCTURAL PROPERTIES OF MEMBRANES

膜的结构特性

• 批准号: 2215004
• 负责人: ROBERT BITTMAN
• 金额: $ 23.98万
• 依托单位: QUEENS COLLEGE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2215004
• 关键词: Saccharomyces; chemical chain length; chemical structure function; cholesterol; fluorescence polarization; glycerol; high performance liquid chromatography; lipid transport; low density lipoprotein; membrane lipids; membrane model; membrane permeability; membrane proteins; membrane reconstitution /synthesis; membrane structure; pheromone; phosphatidylcholines; phospholipids; radiotracer; sphingomyelins; sterols; stop flow technique; temperature jump

In order to improve our understanding at the molecular level of how lipid organization determines membrane function, experiments are described to analyze lipid-lipid and lipid-protein interactions using spectral, kinetic, and permeability methods. For many of these studies, a series of phosphatidylcholine and sphingomyelin analogs will be synthesized. Special attention will be given to the preparation of compounds differing in the structure of the interfacial region. These analogs will be used to examine the importance of specific sites in the interactions between phospholipids, cholesterol, and proteins and in the rates of lipid movement between membranes. The properties of unusual, naturally occurring ether-linked phospholipids will also be studied. These studies are relevant to an understanding of the structures of both normal membranes and of membranes associated with several disease processes, such as atherosclerosis, where lipid accumulation takes place.

为了提高我们在分子水平上的理解 组织确定膜功能，将实验描述为 使用光谱，动力学，分析脂质脂质和脂质 - 蛋白质相互作用 和渗透率方法。 对于许多研究，一系列 磷脂酰胆碱和鞘磷脂类似物将合成。 特别的 将注意化合物的制备不同 界面区域的结构。 这些类似物将用于检查 特定位点在磷脂之间相互作用中的重要性， 胆固醇和蛋白质以及脂质运动速率 膜。 不寻常的，天然发生的醚链接的特性 还将研究磷脂。 这些研究与 了解正常膜和膜的结构 与几种疾病过程相关，例如动脉粥样硬化，其中 脂质积累发生。

项目成果

Effects of sterols on permeability and phase transitions of bilayers from phosphatidylcholines lacking acyl groups.

甾醇对缺乏酰基的磷脂酰胆碱双层的渗透性和相变的影响。

• DOI: 10.1021/bi00513a013
• 发表时间: 1981
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Bittman,R;Clejan,S;Jain,MK;Deroo,PW;Rosenthal,AF
• 通讯作者: Rosenthal,AF

Determination of cholesterol asymmetry by rapid kinetics of filipin-cholesterol association: effect of modification in lipids and proteins.

通过菲律宾胆固醇关联的快速动力学测定胆固醇不对称性：脂质和蛋白质修饰的影响。

• DOI: 10.1021/bi00512a009
• 发表时间: 1981
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Bittman,R;Blau,L;Clejan,S;Rottem,S
• 通讯作者: Rottem,S

Differential scanning calorimetry study of the influence of phospholipid analogs with a carbonyl-terminated sn-2 chain on the interdigitated phases formed by 1-stearoyl-2-capryl-sn-glycero-3-phosphatidylcholine (C18:C10-PC).

差示扫描量热法研究具有羰基末端 sn-2 链的磷脂类似物对 1-硬脂酰-2-辛基-sn-甘油-3-磷脂酰胆碱 (C18:C10-PC) 形成的叉指相的影响。

• 发表时间: 1996
• 期刊: Journal of lipid research
• 影响因子: 6.5
• 作者: Ali,S;Bittman,R
• 通讯作者: Bittman,R

Total synthesis of two photoactivatable analogues of the growth-factor-like mediator sphingosine 1-phosphate: differential interaction with protein targets.

• DOI: 10.1021/jo034828q
• 发表时间: 2003-08
• 期刊: The Journal of organic chemistry
• 作者: Xuequan Lu;S. Cseh;H. Byun;G. Tigyi;R. Bittman

Kinetics of cholesterol and phospholipid exchange between mycoplasma membranes and lipid vesicles.

• 发表时间: 1987-05
• 期刊: Israel journal of medical sciences
• 作者: R. Bittman;S. Clejan