CYTOKINE/ADHESION MOLECULE LIPOSOMES AS HIV ADJUVANTS

细胞因子/粘附分子脂质体作为 HIV 佐剂

• 批准号: 3548036
• 负责人: Lawrence B Lachman
• 金额: $ 18.53万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3548036
• 关键词: AIDS vaccines; HIV envelope protein gp120; HIV envelope protein gp41; active immunization; antibody formation; drug design /synthesis /production; enzyme linked immunosorbent assay; human immunodeficiency virus; immunomodulators; interleukin 1; interleukin 6; laboratory mouse; laboratory rabbit; leukocyte adhesion molecules; liposomes; tissue /cell culture; tumor necrosis factor alpha; virus antigen

The AIDS crisis has demonstrated the immediate need for adjuvants that can induce lasting immunity. Although clinical trials have begun with several of the well characterized antigens from Human Immunodeficiency Virus (HIV), none have yet induced levels of immunity necessary to induce protection from the virus. The research objective of this proposal is to determine if liposomes that express on their exterior surface HIV antigens, macrophage-derived immunological mediators, macrophage activating agents or lymphocyte adhesion molecules can be used to develop a subunit vaccine for AIDS. The specific aims of this proposal are: 1. To determine if liposomes which express on their exterior surface recombinant IL 1, TNF, IL 6 and HIV antigens can induce immunity in mice and rabbits. 2. To determine if cellular adhesion molecules will increase the immunogenicity of the liposomes described above by targeting them to lymphocytes and endothelial venules of lymph nodes. 3. To determine if the macrophage activating agent CGP 31362 will increase the adjuvanticity of different combinations of the liposomes described above.

艾滋病危机表明，迫切需要佐剂， 可以产生持久的免疫力。 虽然临床试验已经开始， 几种来自人类免疫缺陷病毒的充分表征的抗原 病毒（艾滋病毒），还没有诱导免疫水平所需的诱导 保护免受病毒。 这项建议的研究目的是 确定在其外表面表达HIV的脂质体 抗原，巨噬细胞源性免疫介质，巨噬细胞 活化剂或淋巴细胞粘附分子可用于开发 艾滋病的亚单位疫苗 这项建议的具体目标是： 1.为了确定在其外表面表达的脂质体是否 重组IL-1、TNF、IL-6和HIV抗原均可诱导小鼠产生免疫应答 还有兔子 2.为了确定细胞粘附分子是否会增加 通过将它们靶向于 淋巴结的淋巴细胞和内皮微静脉。 3.为了确定巨噬细胞活化剂CGP 31362是否会 增加脂质体的不同组合的佐剂性 上面描述