BIOLOGICAL STUDIES OF HUMAN INTERLEUKIN 1

人白细胞介素 1 的生物学研究

• 批准号: 3176054
• 负责人: Lawrence B Lachman
• 金额: $ 15.36万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1992-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176054
• 关键词: affinity chromatography; angiogenesis; biological response modifiers; drug delivery systems; enzyme linked immunosorbent assay; gel electrophoresis; genetic translation; high performance liquid chromatography; histocompatibility antigens; human tissue; inflammation; interleukin 1; interleukin 2; laboratory mouse; leukocyte activation /transformation; liposomes; macrophage; membrane proteins; messenger RNA; metastasis; neoplasm /cancer immunotherapy; protein structure; synthetic peptide

The specific aims of this proposal are: 1) To determine if liposomes containing Interleukin 1 on their outer membranes may be used as synthetic macrophages to present antigen to lymphocytes, initiate the lymphokine cascade, kill IL 1-sensitive tumor cells, and stimulate the growth of IL 1- responsive cells. 2) To evaluate the biological activity of IL 1-liposomes in vivo for the ability to initiate the sequelae of inflammation, to induce angiogenesis, and to inhibit the growth and metastasis of tumors. 3) To elucidate the molecular mechanisms of macrophage activation which lead to induction and translation of IL 1-specific mRNA and to evaluate the effects of specific inhibitors of macrophage activation. 4) To determine the series of post-translational intracellular events which lead to the secretion of IL 1 from the monocyte and the deposition and anchoring of IL 1 in the membrane. 5) To further evaluate synthetic peptides which have been demonstrated to inhibit the biological activity of human IL 1.

这项建议的具体目标是： 1)为了确定脂质体上是否含有白细胞介素1， 外膜可用作合成巨噬细胞， 将抗原呈递给淋巴细胞，启动淋巴因子级联反应， 杀死IL 1敏感的肿瘤细胞，并刺激IL 1- 反应细胞 2)评价IL-1脂质体的体内生物学活性， 引发炎症后遗症的能力， 血管生成，并抑制肿瘤的生长和转移。 3)阐明巨噬细胞的分子机制 激活，导致诱导和翻译IL 1特异性 mRNA的表达，并评估特异性抑制剂的作用， 巨噬细胞活化 4)为了确定一系列的翻译后细胞内 导致单核细胞分泌IL 1的事件， IL 1在膜上的沉积和锚定。 5)为了进一步评估已经被 证实抑制人IL 1的生物活性。