CYTOKINE/ADHESION MOLECULE LIPOSOMES AS HIV ADJUVANTS

艾滋病危机表明了对佐剂的迫切需求 可以诱导持久的免疫力。尽管临床试验已经开始， 几种具有良好特性的人类免疫缺陷抗原 病毒(HIV)，还没有诱导出必要的免疫水平 保护免受病毒侵袭。这项建议的研究目标是 确定在其外表面表达的脂质体是否为HIV 抗原、巨噬细胞源性免疫介质、巨噬细胞 可以使用激活剂或淋巴细胞黏附分子来开发 一种用于艾滋病的亚单位疫苗。 这项建议的具体目标是： 1.测定在其外表面表达的脂质体 重组白介素1、肿瘤坏死因子、白介素6和人类免疫缺陷病毒抗原诱导小鼠免疫 还有兔子。 2.确定细胞黏附分子是否会增加 通过靶向上述脂质体的免疫原性 淋巴结内的淋巴细胞和内皮小静脉。 3.确定巨噬细胞激活剂CGp 31362是否会 增加不同脂质体组合的佐剂性 如上所述。

The AIDS crisis has demonstrated the immediate need for adjuvants that can induce lasting immunity. Although clinical trials have begun with several of the well characterized antigens from Human Immunodeficiency Virus (HIV), none have yet induced levels of immunity necessary to induce protection from the virus. The research objective of this proposal is to determine if liposomes that express on their exterior surface HIV antigens, macrophage-derived immunological mediators, macrophage activating agents or lymphocyte adhesion molecules can be used to develop a subunit vaccine for AIDS. The specific aims of this proposal are: 1. To determine if liposomes which express on their exterior surface recombinant IL 1, TNF, IL 6 and HIV antigens can induce immunity in mice and rabbits. 2. To determine if cellular adhesion molecules will increase the immunogenicity of the liposomes described above by targeting them to lymphocytes and endothelial venules of lymph nodes. 3. To determine if the macrophage activating agent CGP 31362 will increase the adjuvanticity of different combinations of the liposomes described above.