DISPLAY OF MRNAS FROM NORMAL TUMORIGENIC CELLS

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Differential gene expression is a highly regulated process which controls normal cell growth and differentiation; its abnormality is the underlying cause of disease states such as cancer. Gen-Hunter corporation's objective is twofold: (1) To provide the biomedical research community with complete line of reagent systems based on the latest technological breakthrough known as Differential Display. (2) To use our expertise to search for early diagnostic markers or therapeutic targets for disease states such as cancer. The reagent systems from GenHunter have made identifications and clonings of differentially expressed genes within easy reach for every researcher. The principle of Differential Display is to systematically distribute mRNA 3' termini on a denaturing polyacrylamide gel after RNA-PCR using multiple combinations of anchored oligo-dT and arbitrary decamers. This proposed Selective Display approach modifies the method by using, instead of anchored oligo-dT primers, a set of gene family-specific decamers in combinations with arbitrary decamers to display only those genes of interest. Thus it complements Differential Display by narrowing down the scope of a gene search while allowing only the coding regions of mRNAs to be displayed. If successful, this work will lead to more lines of commercial reagent systems for the general research community and will greatly speed up hunting down many important disease-causing genes.